Overview

  • Product name

  • Description

    Rabbit polyclonal to PABPC5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 12-267 of Human PABPC5 (UniProt Q96DU9).

  • Positive control

    • U87-MG, NT2D1, IMR32 and MCF-7 whole cell lysates, Human gastric cancer tissue.
  • General notes

    Store as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab151459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval buffer: Tris-EDTA buffer (pH8.0).

Target

  • Relevance

    PABPC5 (polyadenylate-binding protein 5) binds the polyadenylate tail of mRNA and is thought to be involved in cytoplasmic regulatory processes of mRNA metabolism. In vivo, it may also bind to cytoplasmic RNA sequences other than polyadenylate.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • PABP5 antibody
    • Poly(A) binding protein 5 antibody
    • Poly(A) binding protein cytoplasmic 5 antibody
    • Polyadenylate binding protein 5 antibody

Images

  • Anti-PABPC5 antibody (ab151459) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Gastric cancer tissue labelling PABPC5 with ab151459 at 1/500 dilution.

References

ab151459 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151459.
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