Key features and details
- Rabbit polyclonal to PABPC5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PABPC5 antibody
See all PABPC5 primary antibodies
DescriptionRabbit polyclonal to PABPC5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Lar gibbon
- WB: PABPC5 overexpression HEK-293T lysate. IHC-P: Human cerebral cortex tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevancePABPC5 (polyadenylate-binding protein 5) binds the polyadenylate tail of mRNA and is thought to be involved in cytoplasmic regulatory processes of mRNA metabolism. In vivo, it may also bind to cytoplasmic RNA sequences other than polyadenylate.
- PABP5 antibody
- Poly(A) binding protein 5 antibody
- Poly(A) binding protein cytoplasmic 5 antibody
- Polyadenylate binding protein 5 antibody
Paraffin-embedded human cerebral cortex tissue stained for PABPC5 using ab224208 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-PABPC5 antibody (ab224208) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with a large T antigen) cell lysate
Lane 2 : PABPC5 overexpression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Developed using the ECL technique.
Predicted band size: 43 kDa
ab224208 has not yet been referenced specifically in any publications.