Overview

  • Product name

  • Description

    Goat polyclonal to PACAP
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human PACAP aa 159-172 (C terminal) (Cysteine residue). NP_001108.2
    Sequence:

    C-GKRYKQRVKNKGRR


    Database link: P18509

  • Positive control

    • WB: Human hippocampus lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 19 kDa).

Target

  • Function

    Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca(2+) stores, antibody secretion and integrin activation.
    Acts as a hormone-regulated adipokine/proinflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.
  • Tissue specificity

    Widely expressed with highest levels in adult brain, small intestine and lymphoid tissues such as thymus and spleen. Expression is frequently lower in intestinal-type gastric cancer. In obese patients, more abundant in omental than in subcutaneous fat.
  • Sequence similarities

    Belongs to the MZB1 family.
  • Post-translational
    modifications

    Forms an interchain disulfide bond with IgM monomers.
  • Cellular localization

    Endoplasmic reticulum lumen. Secreted and Cytoplasm. Diffuse granular localization in the cytoplasm surrounding the nucleus (PubMed:11350957).
  • Information by UniProt
  • Database links

  • Alternative names

    • MZB1 antibody
    • Caspase 2 binding protein antibody
    • FLJ32987 antibody
    • HSPC190 antibody
    • Marginal zone B and B1 cell specific protein antibody
    • Marginal zone B- and B1-cell-specific protein antibody
    • MEDA 7 antibody
    • MEDA-7 antibody
    • Mesenteric estrogen dependent adipose 7 antibody
    • Mesenteric estrogen-dependent adipose 7 antibody
    • Mesenteric oestrogen dependent adipose gene 7 antibody
    • MGC29506 antibody
    • Mzb1 antibody
    • MZB1_HUMAN antibody
    • PACAP antibody
    • pERp1 antibody
    • Plasma cell-induced ER protein 1 antibody
    • Plasma cell-induced resident endoplasmic reticulum protein antibody
    • Plasma cell-induced resident ER protein antibody
    • Proapoptotic caspase adapter protein antibody
    see all

Images

  • Anti-PACAP antibody (ab223668) at 1 mg/ml + human hippocampus lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 19 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour

References

ab223668 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (30 Micron Brain Sections)
Permeabilization
No
Specification
30 Micron Brain Sections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 11 2018

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