Overview

  • Product name

  • Description

    Rabbit polyclonal to PACAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PACAP aa 1-189.

  • Positive control

    • MOLT4 or Raji cell lysates
  • General notes

     This product was previously labelled as Proapoptotic Caspase Adaptor Protein

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.

Target

  • Function

    Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca(2+) stores, antibody secretion and integrin activation.
    Acts as a hormone-regulated adipokine/proinflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.
  • Tissue specificity

    Widely expressed with highest levels in adult brain, small intestine and lymphoid tissues such as thymus and spleen. Expression is frequently lower in intestinal-type gastric cancer. In obese patients, more abundant in omental than in subcutaneous fat.
  • Sequence similarities

    Belongs to the MZB1 family.
  • Post-translational
    modifications

    Forms an interchain disulfide bond with IgM monomers.
  • Cellular localization

    Endoplasmic reticulum lumen. Secreted and Cytoplasm. Diffuse granular localization in the cytoplasm surrounding the nucleus (PubMed:11350957).
  • Information by UniProt
  • Database links

  • Alternative names

    • MZB1 antibody
    • Caspase 2 binding protein antibody
    • FLJ32987 antibody
    • HSPC190 antibody
    • Marginal zone B and B1 cell specific protein antibody
    • Marginal zone B- and B1-cell-specific protein antibody
    • MEDA 7 antibody
    • MEDA-7 antibody
    • Mesenteric estrogen dependent adipose 7 antibody
    • Mesenteric estrogen-dependent adipose 7 antibody
    • Mesenteric oestrogen dependent adipose gene 7 antibody
    • MGC29506 antibody
    • Mzb1 antibody
    • MZB1_HUMAN antibody
    • PACAP antibody
    • pERp1 antibody
    • Plasma cell-induced ER protein 1 antibody
    • Plasma cell-induced resident endoplasmic reticulum protein antibody
    • Plasma cell-induced resident ER protein antibody
    • Proapoptotic caspase adapter protein antibody
    see all

Images

  • All lanes : Anti-PACAP antibody (ab96308) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 21 kDa



    15% SDS PAGE

References

This product has been referenced in:

  • Kocevar N  et al. Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers. World J Gastroenterol 18:1216-28 (2012). WB ; Human . Read more (PubMed: 22468085) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (Stomach)
Loading amount
30 µg
Specification
Stomach
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 13 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up