Key features and details
- Rabbit polyclonal to PACAP receptor/ADCYAP1R1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PACAP receptor/ADCYAP1R1 antibody
See all PACAP receptor/ADCYAP1R1 primary antibodies
DescriptionRabbit polyclonal to PACAP receptor/ADCYAP1R1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Cow
Synthetic peptide corresponding to Human PACAP receptor/ADCYAP1R1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Human brain; Human liver; Human pancreas; Human small intestine.
This product was previously labelled as PACAP receptor
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab95278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
FunctionThis is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.
Tissue specificityMost abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
Cellular localizationCell membrane.
- Information by UniProt
- ADCYAP1 R1 antibody
- ADCYAP1R 1 antibody
- ADCYAP1R1 antibody
All lanes : Anti-PACAP receptor/ADCYAP1R1 antibody (ab95278) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 51 kDa (possible cleavage fragment)
Exposure time: 10 minutes
The band observed at 51 kDa could potentially be a cleaved form of PACAP receptor/ADCYAP1R1 due to the presence of a 20 amino acid signal peptide. A positive signal was also seen in both Human liver and Human pancreas tissue lysates.
ab95278 has not yet been referenced specifically in any publications.