Key features and details
- Rabbit polyclonal to PACE4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PACE4 antibody
See all PACE4 primary antibodies
DescriptionRabbit polyclonal to PACE4
SpecificityBLAST analysis of the peptide immunogen showed no homology with other Human proteins.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Monkey, Gorilla, Opossum, Common marmoset, Elephant
Synthetic peptide of 16 amino acid from internal region of Human PACE (UniProt ID: P29122; NP_002561.1).
- Human brain tissue (cortex)
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab140934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 - 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionLikely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Tissue specificityEach PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
Sequence similaritiesBelongs to the peptidase S8 family.
Contains 1 homo B/P domain.
Contains 1 PLAC domain.
DomainThe propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
Cellular localizationSecreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
- Information by UniProt
- Paired basic amino acid cleaving enzyme 4 antibody
- Paired basic amino acid cleaving system 4 antibody
- PCSK6 antibody
ab140934 has been referenced in 1 publication.
- Zhang KK et al. Gene network and familial analyses uncover a gene network involving Tbx5/Osr1/Pcsk6 interaction in the second heart field for atrial septation. Hum Mol Genet 25:1140-51 (2016). PubMed: 26744331