Overview

  • Product name

  • Description

    Rabbit polyclonal to PACE4
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Monkey, Gorilla, Opossum, Common marmoset, Elephant
  • Immunogen

    Synthetic peptide of 16 amino acid from internal region of Human PACE (UniProt ID: P29122; NP_002561.1).

  • Positive control

    • Human brain tissue (cortex)

Properties

Applications

Our Abpromise guarantee covers the use of ab140934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 - 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
  • Tissue specificity

    Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
  • Sequence similarities

    Belongs to the peptidase S8 family.
    Contains 1 homo B/P domain.
    Contains 1 PLAC domain.
  • Domain

    The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
  • Cellular localization

    Secreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Paired basic amino acid cleaving enzyme 4 antibody
    • Paired basic amino acid cleaving system 4 antibody
    • PCSK6 antibody
    • PCSK6_HUMAN antibody
    • Proprotein convertase subtilisin/kexin type 6 antibody
    • SPC4 antibody
    • Subtilisin like protease antibody
    • Subtilisin-like proprotein convertase 4 antibody
    • subtilisin/kexin like protease PACE4 antibody
    • Subtilisin/kexin-like protease PACE4 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue (cortex) labelling PACE4 with ab140934 at 15 µg/ml.

References

This product has been referenced in:

  • Zhang KK  et al. Gene network and familial analyses uncover a gene network involving Tbx5/Osr1/Pcsk6 interaction in the second heart field for atrial septation. Hum Mol Genet 25:1140-51 (2016). Read more (PubMed: 26744331) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart, liver, pancreas, intestine, colon, kidney,)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
100 µg
Specification
heart, liver, pancreas, intestine, colon, kidney,
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Linglin Xie

Verified customer

Submitted Apr 16 2019

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