Overview

  • Product name

    Anti-PACE4 antibody [EPR8320]
    See all PACE4 primary antibodies
  • Description

    Rabbit monoclonal [EPR8320] to PACE4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PACE4 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • HeLa and HepG2 whole cell lysate (ab7900); HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000 - 1/10000. Predicted molecular weight: 106 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
    • Tissue specificity

      Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
    • Sequence similarities

      Belongs to the peptidase S8 family.
      Contains 1 homo B/P domain.
      Contains 1 PLAC domain.
    • Domain

      The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
    • Cellular localization

      Secreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
    • Information by UniProt
    • Database links

    • Alternative names

      • Paired basic amino acid cleaving enzyme 4 antibody
      • Paired basic amino acid cleaving system 4 antibody
      • PCSK6 antibody
      • PCSK6_HUMAN antibody
      • Proprotein convertase subtilisin/kexin type 6 antibody
      • SPC4 antibody
      • Subtilisin like protease antibody
      • Subtilisin-like proprotein convertase 4 antibody
      • subtilisin/kexin like protease PACE4 antibody
      • Subtilisin/kexin-like protease PACE4 antibody
      see all

    Images

    • All lanes : Anti-PACE4 antibody [EPR8320] (ab151562) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 106 kDa

    • ab151562 staining HeLa cells by ICC/IF. The cells were fixed with methanol. The cells were then stained with ab151562 at 1/200 in PBS for 1h at 22°C. An Alexa Fluro 488 goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. ab151562 produces a focal staining pattern that is enriched within the cytoplasm.

      See Abreview

    • Flow cytometric analysis of permeabilized HeLa cells labeling PACE4 with ab151562 at 1/100 dilution (red), compared to a Rabbit IgG control antibody (green).

    References

    This product has been referenced in:

    • Chen S  et al. Functional analysis of corin protein domains required for PCSK6-mediated activation. Int J Biochem Cell Biol 94:31-39 (2018). WB . Read more (PubMed: 29180304) »
    • Tian XF  et al. PACE4 regulates apoptosis in human pancreatic cancer Panc-1 cells via the mitochondrial signaling pathway. Mol Med Rep 14:5205-5210 (2016). Read more (PubMed: 27779720) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    50 µg
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Emad Khoury

    Verified customer

    Submitted Jun 14 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    No
    Fixative
    Methanol

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 29 2013

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