Overview

  • Product name
    Paclitaxel, Anticancer agent
  • Description
    Anticancer agent
  • Alternative names
    • Taxol
  • Biological description

    Anticancer agent. Inhibits depolymerization of microtubules, causing mitotic arrest in cancer cells, and apoptosis.

  • Purity
    > 98%
  • CAS Number
    33069-62-4
  • Chemical structure
    Chemical Structure

Properties

Images

  • E2 inhibits Paclitaxel induced androgen-independent prostate cancer cell death.

    (A-D) 100 nM of E2 was added to the media of (A and B) LNCaP and (C and D) PC3 cells for 96h, followed by addition of Paclitaxel at the indicated concentrations for 24h. The cells were stained with Hoechst 33258 (5 µg/ml) to visualize nuclei and propidium iodide (PI) (0.2 µg/ml) to detect membrane damage (B and D). Cell death was quantified by scoring the number of PI positive cells relative to the total number cell nuclei in the same visual field (A and C). The values represent the mean ± S.E. of at least three independent experiments. * denotes p<0.05, ** denotes p<0.01, and *** denotes p<0.001.

    E2 = 17-β-estradiol

    (From Figure 1 of Dong et al).

References

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Abreviews
Microtubules (MT) were isolated from SH-SY5y cells using protocol outlined in: Sloboda RD. 2014b. Isolation of microtubules and microtubule-associated proteins using paclitaxel. Cold Spring Harb Protoc doi: 10.1101/pdb .prot081190.
Following isolation, 20 μg of protein from the MT extract was immunoblotted for detecting B-3-Tubulin (Primary antibody - 1:500 dilution overnight, Secondary antibody (ab6728) - 1: 2000 dilution for 1h).

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Submitted Feb 28 2018

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