Key features and details
- Rabbit polyclonal to PACRG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
Our Abpromise guarantee covers the use of ab4090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).|
FunctionSuppresses cell death induced by accumulation of unfolded Pael receptor (Pael-R, a substrate of Parkin). Facilitates the formation of inclusions consisting of Pael-R, molecular chaperones, protein degradation molecules and itself when proteasome is inhibited. May play an important role in the formation of Lewy bodies and protection of dopaminergic neurons against Parkinson disease.
Tissue specificityExpressed in all immune tissues, spleen, lymph nodes, thymus, tonsils, leukocyte and bone marrow. Expressed also in heart, brain, skeletal muscle, kidney, lung and pancreas. Expressed in primary Schwann cells and very weakly by monocyte-derived macrophages the primary host cells of Mycobacterium leprae, the causative agent of leprosy. Component of Lewy bodies, intraneuronal inclusions found in the brain of Parkinson disease patients.
- Information by UniProt
- FLJ32724 antibody
- GLUP antibody
- HAK005771 antibody
All lanes : Anti-PACRG antibody (ab4090) at 1/1500 dilution
Lane 1 : Mouse embryonic fibroblast (MEF) whole cell lysate
Lane 2 : Mouse embryonic fibroblast (MEF) whole cell lysate with immunising peptide
Lysates/proteins at 35 µg per lane.
All lanes : IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
ab4090 has been referenced in 1 publication.
- Gavino C & Richard S Patched1 haploinsufficiency impairs ependymal cilia function of the quaking viable mice, leading to fatal hydrocephalus. Mol Cell Neurosci 47:100-7 (2011). PubMed: 21447392