Key features and details
- Rabbit polyclonal to PACS1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PACS1 antibody
See all PACS1 primary antibodies
DescriptionRabbit polyclonal to PACS1
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human cerebral cortex, lymph node and testis tissue. ICC/IF: A431 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionCoat protein that is involved in the localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin-coated membrane pits. Involved in HIV-1 nef-mediated removal of MHC-I from the cell surface to the TGN.
Involvement in diseaseMental retardation, autosomal dominant 17
Sequence similaritiesBelongs to the PACS family.
Cellular localizationGolgi apparatus, trans-Golgi network. Localizes in the perinuclear region, probably the TGN.
- Information by UniProt
- Cytosolic sorting protein PACS1 antibody
- PACS 1 antibody
- PACS-1 antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling PACS1 (Green) using ab243703 at 4 µg/mL in ICC/IF analysis.
Formalin-fixed, paraffin-embedded human testis tissue stained for PACS1 using ab243703 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human lymph node tissue stained for PACS1 using ab243703 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for PACS1 using ab243703 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for PACS1 using ab243703 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243703 has not yet been referenced specifically in any publications.