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  1. Link

    pacsin1-antibody-ab89604.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Anti-PACSIN1 antibody (ab89604)

  • Datasheet
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Western blot - Anti-PACSIN1 antibody (ab89604)
  • Western blot - Anti-PACSIN1 antibody (ab89604)

Key features and details

  • Mouse polyclonal to PACSIN1
  • Suitable for: WB
  • Reacts with: Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-PACSIN1 antibody
    See all PACSIN1 primary antibodies
  • Description

    Mouse polyclonal to PACSIN1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Full length protein corresponding to amino acids 1-444 of Human PACSIN1 (NP_065855.1).

  • Positive control

    • Rat brain lysate, PACSIN1 transfected 293T cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)

Applications

Our Abpromise guarantee covers the use of ab89604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.

Target

  • Function

    May play a role in vesicle formation and transport.
  • Tissue specificity

    Highly expressed in brain and in a lesser extent in heart, pancreas and liver.
  • Sequence similarities

    Belongs to the PACSIN family.
    Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
  • Cellular localization

    Cytoplasm. Colocalizes with dynamin I at vesicular structures in the cell body and neurites.
  • Target information above from: UniProt accession Q9BY11 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29993 Human
    • Entrez Gene: 29704 Rat
    • Omim: 606512 Human
    • SwissProt: Q9BY11 Human
    • SwissProt: Q9Z0W5 Rat
    • Unigene: 520087 Human
    • Unigene: 161729 Rat
    • Alternative names

      • A830061D09Rik antibody
      • Dynamin PRD-interacting protein antibody
      • Dynamin proline-rich domain-interacting protein antibody
      • H74 antibody
      • KIAA1379 antibody
      • MGC114042 antibody
      • MGC56324 antibody
      • mKIAA1379 antibody
      • OTTHUMP00000016231 antibody
      • PACN1_HUMAN antibody
      • Pacsin1 antibody
      • PASCIN antibody
      • Protein kinase C and casein kinase substrate in neurons 1 antibody
      • Protein kinase C and casein kinase substrate in neurons protein 1 antibody
      • SDPI antibody
      • Synaptic, dynamin-associated protein I antibody
      • Syndapin 1 antibody
      • Syndapin antibody
      • Syndapin-I antibody
      • zgc:114042 antibody
      • zgc:56324 antibody
      see all

    Images

    • Western blot - Anti-PACSIN1 antibody (ab89604)
      Western blot - Anti-PACSIN1 antibody (ab89604)
      Anti-PACSIN1 antibody (ab89604) at 1 µg/ml + Rat brain lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 51 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-PACSIN1 antibody (ab89604)
      Western blot - Anti-PACSIN1 antibody (ab89604)
      All lanes : Anti-PACSIN1 antibody (ab89604) at 1 µg/ml

      Lane 1 : PACSIN1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 51 kDa
      Observed band size: 51 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab89604? Please let us know so that we can cite the reference in this datasheet.

    ab89604 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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