Anti-PACSIN1 antibody (ab89604)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PACSIN1 antibody
See all PACSIN1 primary antibodies -
Description
Mouse polyclonal to PACSIN1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length protein corresponding to amino acids 1-444 of Human PACSIN1 (NP_065855.1).
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Positive control
- Rat brain lysate, PACSIN1 transfected 293T cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab89604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Function
May play a role in vesicle formation and transport. -
Tissue specificity
Highly expressed in brain and in a lesser extent in heart, pancreas and liver. -
Sequence similarities
Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). -
Cellular localization
Cytoplasm. Colocalizes with dynamin I at vesicular structures in the cell body and neurites. - Information by UniProt
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Database links
- Entrez Gene: 29993 Human
- Entrez Gene: 29704 Rat
- Omim: 606512 Human
- SwissProt: Q9BY11 Human
- SwissProt: Q9Z0W5 Rat
- Unigene: 520087 Human
- Unigene: 161729 Rat
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Alternative names
- A830061D09Rik antibody
- Dynamin PRD-interacting protein antibody
- Dynamin proline-rich domain-interacting protein antibody
see all
Images
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Western blot - Anti-PACSIN1 antibody (ab89604)Anti-PACSIN1 antibody (ab89604) at 1 µg/ml + Rat brain lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PACSIN1 antibody (ab89604)All lanes : Anti-PACSIN1 antibody (ab89604) at 1 µg/ml
Lane 1 : PACSIN1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Protocols
Datasheets and documents
References
ab89604 has not yet been referenced specifically in any publications.
