Anti-PACSIN1 antibody (ab89604)
Key features and details
- Mouse polyclonal to PACSIN1
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PACSIN1 antibody
See all PACSIN1 primary antibodies -
Description
Mouse polyclonal to PACSIN1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length protein corresponding to amino acids 1-444 of Human PACSIN1 (NP_065855.1).
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Positive control
- Rat brain lysate, PACSIN1 transfected 293T cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab89604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Function
May play a role in vesicle formation and transport. -
Tissue specificity
Highly expressed in brain and in a lesser extent in heart, pancreas and liver. -
Sequence similarities
Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). -
Cellular localization
Cytoplasm. Colocalizes with dynamin I at vesicular structures in the cell body and neurites. - Information by UniProt
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Database links
- Entrez Gene: 29993 Human
- Entrez Gene: 29704 Rat
- Omim: 606512 Human
- SwissProt: Q9BY11 Human
- SwissProt: Q9Z0W5 Rat
- Unigene: 520087 Human
- Unigene: 161729 Rat
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Alternative names
- A830061D09Rik antibody
- Dynamin PRD-interacting protein antibody
- Dynamin proline-rich domain-interacting protein antibody
see all
Images
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Anti-PACSIN1 antibody (ab89604) at 1 µg/ml + Rat brain lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PACSIN1 antibody (ab89604) at 1 µg/ml
Lane 1 : PACSIN1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Datasheets and documents
References (0)
ab89604 has not yet been referenced specifically in any publications.