Product nameAnti-PACSIN2 antibody
DescriptionRabbit polyclonal to PACSIN2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human PACSIN2 (internal sequence). The exact sequence is proprietary.
Database link: Q9UNF0
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionMay play a role in vesicle formation and transport.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.
modificationsPhosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Cellular localizationCytoplasmic vesicle. Vesicle-like cytoplasmic distribution.
- Information by UniProt
- cytoplasmic phosphoprotein PACSIN2 antibody
- OTTHUMP00000198281 antibody
- OTTHUMP00000198282 antibody
All lanes : Anti-PACSIN2 antibody (ab228589) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 56 kDa
Paraffin-embedded human colon carcinoma tissue stained for PACSIN2 with ab228589 at 1/500 dilution in immunohistochemical analysis
ab228589 has not yet been referenced specifically in any publications.