Overview

  • Product name

  • Description

    Rabbit polyclonal to PACSIN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PACSIN2 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UNF0

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Human colon carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    May play a role in vesicle formation and transport.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the PACSIN family.
    Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
  • Cellular localization

    Cytoplasmic vesicle. Vesicle-like cytoplasmic distribution.
  • Information by UniProt
  • Database links

  • Alternative names

    • cytoplasmic phosphoprotein PACSIN2 antibody
    • OTTHUMP00000198281 antibody
    • OTTHUMP00000198282 antibody
    • OTTHUMP00000198283 antibody
    • OTTHUMP00000198284 antibody
    • PACN2_HUMAN antibody
    • Pacsin2 antibody
    • protein kinase C and casein kinase substrate in neurons 2 antibody
    • Protein kinase C and casein kinase substrate in neurons protein 2 antibody
    • SDPII antibody
    • Syndapin II antibody
    see all

Images

  • All lanes : Anti-PACSIN2 antibody (ab228589) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 56 kDa



    10% SDS-PAGE

  • Paraffin-embedded human colon carcinoma tissue stained for PACSIN2 with ab228589 at 1/500 dilution in immunohistochemical analysis

References

ab228589 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon)
Permeabilization
Yes - Ethanol
Specification
Colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol

Ms. Anna Southworth

Verified customer

Submitted Jan 15 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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