Anti-PADI2 / PAD2 antibody (ab16478)
Key features and details
- Rabbit polyclonal to PADI2 / PAD2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PADI2 / PAD2 antibody
See all PADI2 / PAD2 primary antibodies -
Description
Rabbit polyclonal to PADI2 / PAD2 -
Host species
Rabbit -
Specificity
ab16478 recognises a specific 75kDa band corresponding to PADI2, which is specifically blocked using the immunizing peptide in human colon, skeletal muscle and kidney lysates. There is a non-specific 18kDa band present in skeletal muscle lysates, which is attributed to cross-reactivity of the PADI2 antibody
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Tested Applications & Species
Application Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human PADI2/ PAD2 aa 100-200 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17091) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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WB |
Human
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All applications |
Rat
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Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa).
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa). |
Target
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Function
Catalyzes the deimination of arginine residues of proteins. -
Sequence similarities
Belongs to the protein arginine deiminase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 11240 Human
- Entrez Gene: 29511 Rat
- Omim: 607935 Human
- SwissProt: Q9Y2J8 Human
- SwissProt: P20717 Rat
- Unigene: 33455 Human
- Unigene: 98523 Rat
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Alternative names
- KIAA0994 antibody
- OTTHUMP00000044625 antibody
- PAD 2 antibody
see all
Images
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Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab16478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. The 75-kDa band observed is consistent with what has been described in the literature (PMID:18668562; 20668670; 16723463).
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ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
References (13)
ab16478 has been referenced in 13 publications.
- Casanova V et al. Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection. Front Immunol 11:85 (2020). PubMed: 32117246
- Gao BS et al. Peptidyl Arginine Deiminase, Type II (PADI2) Is Involved in Urothelial Bladder Cancer. Pathol Oncol Res N/A:N/A (2019). PubMed: 31267364
- García-Berrocoso T et al. Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics 17:175-189 (2018). PubMed: 29133510
- Zhou Y et al. Spontaneous Secretion of the Citrullination Enzyme PAD2 and Cell Surface Exposure of PAD4 by Neutrophils. Front Immunol 8:1200 (2017). PubMed: 28993780
- McNee G et al. Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma. Leukemia 31:373-381 (2017). PubMed: 27400413