• Product name
    Anti-PADI2 / PAD2 antibody
    See all PADI2 / PAD2 primary antibodies
  • Description
    Rabbit polyclonal to PADI2 / PAD2
  • Host species
  • Specificity
    ab16478 recognises a specific 43kDa band corresponding to PADI2, which is specifically blocked using the immunizing peptide in human colon, skeletal muscle and kidney lysates. There is a non-specific 18kDa band present in skeletal muscle lysates, which is attributed to cross-reactivity of the PADI2 antibody
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PADI2/ PAD2.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in the following whole tissue lysates: Human Kidney Normal.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Rectum Normal.

      This antibody gave a positive signal in the following cell lines: HEK293; Human Peripheral Blood Mononuclear cells.



Our Abpromise guarantee covers the use of ab16478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. PubMed: 19085382
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa).


  • Function
    Catalyzes the deimination of arginine residues of proteins.
  • Sequence similarities
    Belongs to the protein arginine deiminase family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0994 antibody
    • OTTHUMP00000044625 antibody
    • PAD 2 antibody
    • PAD H19 antibody
    • PAD-H19 antibody
    • PAD2 antibody
    • PADI 2 antibody
    • Padi2 antibody
    • PADI2 protein antibody
    • PADI2_HUMAN antibody
    • PDI 2 antibody
    • PDI2 antibody
    • Peptidlyarginine deiminase type II antibody
    • Peptidyl arginine deiminase II antibody
    • Peptidyl arginine deiminase type II antibody
    • Peptidylarginine deiminase II antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 2 antibody
    • Protein arginine deiminase type II antibody
    • Protein-arginine deiminase type II antibody
    • Protein-arginine deiminase type-2 antibody
    see all


  • Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 20 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab16478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. The 75-kDa band observed is consistent with what has been described in the literature (PMID:18668562; 20668670; 16723463).

  • Image courtesy of Human Protein Atlas

    ab16478 staining PADI2 in female rectum, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human rectal tissue was incubated with ab16478 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab16478 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab16478 staining human peripheral blood mononuclear cells (cultured with M-CSF) by Flow Cytometery. Cells were treated with flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) and gating was done on myeloid cells. The primary antibody was diluted 1/10 (PBS 0.1% sodium azide 1% BSA) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview


This product has been referenced in:
  • García-Berrocoso T  et al. Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics 17:175-189 (2018). Read more (PubMed: 29133510) »
  • Zhou Y  et al. Spontaneous Secretion of the Citrullination Enzyme PAD2 and Cell Surface Exposure of PAD4 by Neutrophils. Front Immunol 8:1200 (2017). Read more (PubMed: 28993780) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Vielen Dank für Ihre Email.

Leider weiss ich auch nicht genau, was der Unterschied zwischen diesen beiden Banden ist. In UniProt hat es zwei Sequenzen - die annotierte lange Form (http://www.uniprot.org/uniprot/Q9Y2J8) und die noch un- annotierte kurze Form (http://www.uniprot.org/uniprot/Q96DA7).

Da Sie die HEK293 Zellen benutzen, und wir Nierenlysat benutzt haben, kann ich Ihnen vorschlagen, ein gratis Röhrchen von Nierenlysat als positive Kontrolle zu senden. Dann könnten Sie gleich die beiden Proben vergleichen.

Es tut mir leid, Ihnen nicht andersweitig helfen zu können. Es scheint mir, dass die Proteinbiologie von PADI2 noch nicht genau erforscht ist.

Bitte lassen Sie mich wissen, ob Sie dies akzeptieren können und wollen.

Read More
Flow Cytometry
Human Cell (Peripheral blood mononuclear cells)
Peripheral blood mononuclear cells
Cell harvesting/tissue preparation method: Normal human peripheral blood monocytes cultured with M-CSF. Cells were harvested on day 3, non-specific binding was blocked with human Ig, staining was performed on permeabilized cells
Sample buffer: flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) after permeabilization
Caltag Fix Perm Kit
Yes - Caltag Fix Perm Kit
Gating Strategy
Myeloid gate

Dr. Frances Santiago-Schwarz

Verified customer

Submitted Jul 01 2009

Western blot
Human Cell lysate - whole cell (THP-1, MRC-5, human macrophages)
Loading amount
1e+006 cells
THP-1, MRC-5, human macrophages
Blocking step
Other as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Oct 10 2006

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