• Product name
    Anti-PADI2 / PAD2 antibody
    See all PADI2 / PAD2 primary antibodies
  • Description
    Rabbit polyclonal to PADI2 / PAD2
  • Host species
  • Specificity
    ab16478 recognises a specific 43kDa band corresponding to PADI2, which is specifically blocked using the immunizing peptide in human colon, skeletal muscle and kidney lysates. There is a non-specific 18kDa band present in skeletal muscle lysates, which is attributed to cross-reactivity of the PADI2 antibody
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PADI2/ PAD2.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in the following whole tissue lysates: Human Kidney Normal.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Rectum Normal.

      This antibody gave a positive signal in the following cell lines: HEK293; Human Peripheral Blood Mononuclear cells.



Our Abpromise guarantee covers the use of ab16478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. PubMed: 19085382
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa).


  • Function
    Catalyzes the deimination of arginine residues of proteins.
  • Sequence similarities
    Belongs to the protein arginine deiminase family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0994 antibody
    • OTTHUMP00000044625 antibody
    • PAD 2 antibody
    • PAD H19 antibody
    • PAD-H19 antibody
    • PAD2 antibody
    • PADI 2 antibody
    • Padi2 antibody
    • PADI2 protein antibody
    • PADI2_HUMAN antibody
    • PDI 2 antibody
    • PDI2 antibody
    • Peptidlyarginine deiminase type II antibody
    • Peptidyl arginine deiminase II antibody
    • Peptidyl arginine deiminase type II antibody
    • Peptidylarginine deiminase II antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 2 antibody
    • Protein arginine deiminase type II antibody
    • Protein-arginine deiminase type II antibody
    • Protein-arginine deiminase type-2 antibody
    see all


  • Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 20 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab16478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. The 75-kDa band observed is consistent with what has been described in the literature (PMID:18668562; 20668670; 16723463).

  • Image courtesy of Human Protein Atlas

    ab16478 staining PADI2 in female rectum, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human rectal tissue was incubated with ab16478 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab16478 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab16478 staining human peripheral blood mononuclear cells (cultured with M-CSF) by Flow Cytometery. Cells were treated with flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) and gating was done on myeloid cells. The primary antibody was diluted 1/10 (PBS 0.1% sodium azide 1% BSA) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview


This product has been referenced in:
  • Zhou Y  et al. Spontaneous Secretion of the Citrullination Enzyme PAD2 and Cell Surface Exposure of PAD4 by Neutrophils. Front Immunol 8:1200 (2017). Read more (PubMed: 28993780) »
  • McNee G  et al. Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma. Leukemia 31:373-381 (2017). Read more (PubMed: 27400413) »
See all 10 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Flow Cytometry
Human Cell (Peripheral blood mononuclear cells)
Peripheral blood mononuclear cells
Cell harvesting/tissue preparation method: Normal human peripheral blood monocytes cultured with M-CSF. Cells were harvested on day 3, non-specific binding was blocked with human Ig, staining was performed on permeabilized cells
Sample buffer: flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) after permeabilization
Caltag Fix Perm Kit
Yes - Caltag Fix Perm Kit
Gating Strategy
Myeloid gate

Dr. Frances Santiago-Schwarz

Verified customer

Submitted Jul 01 2009

Western blot
Human Cell lysate - whole cell (THP-1, MRC-5, human macrophages)
Loading amount
1e+006 cells
THP-1, MRC-5, human macrophages
Blocking step
Other as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Oct 10 2006

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