Flow Cytometry abreview for Anti-PADI2 / PAD2 antibody

Excellent
Abreviews
Application
Flow Cytometry
Sample
Human Cell (Peripheral blood mononuclear cells)
Specification
Peripheral blood mononuclear cells
Preparation
Cell harvesting/tissue preparation method: Normal human peripheral blood monocytes cultured with M-CSF. Cells were harvested on day 3, non-specific binding was blocked with human Ig, staining was performed on permeabilized cells
Sample buffer: flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) after permeabilization
Fixation
Caltag Fix Perm Kit
Permeabilization
Yes - Caltag Fix Perm Kit
Gating Strategy
Myeloid gate

Other product details

Dilution
1/10
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: PBS 0.1% sodium azide 1% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG1 alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Isotype matched control: Purified Rabbit IgG1

Dr. Frances Santiago-Schwarz

Verified customer

Submitted Jul 01 2009

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