Western blot abreview for Anti-PADI2 / PAD2 antibody

Below Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1, MRC-5, human macrophages)
Loading amount
1e+006 cells
Specification
THP-1, MRC-5, human macrophages
Blocking step
Other as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/250
Incubation time
18 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: IRdye800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Li-Cor
Exposure
30 second(s)
Bands
Specific: 75 kDa Non-specific: 35, 45 kDa
Positive control
Kidney whole cell lysate
Negative control
n/a

Additional data

Abcam response
In your correspondence to us you stated "I seem to be getting low expression levels for several proteins I'm targeting (also confirmed by qPCR). Antibodies to the same targets from other companies have also failed in my experiments. I've used several Abcam antibodies recently against well-expressed proteins and had fantastic results (IRAK4 in particular)". We therefore conclude that the negative results you have experienced are likely to be due to the low levels in your samples. Thank you again for your feedback.

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Verified customer

Submitted Oct 10 2006

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