Product nameAnti-PADI2 / PAD2 antibody
See all PADI2 / PAD2 primary antibodies
DescriptionRabbit polyclonal to PADI2 / PAD2
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Human
Synthetic peptide corresponding to C terminal residues of human PADI2/ PAD2 (peptidyl arginine deiminase, type II)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by antigen specific affinity chromatography.
Our Abpromise guarantee covers the use of ab56075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ELISA||Use at an assay dependent dilution.|
FunctionCatalyzes the deimination of arginine residues of proteins.
Sequence similaritiesBelongs to the protein arginine deiminase family.
- Information by UniProt
- KIAA0994 antibody
- OTTHUMP00000044625 antibody
- PAD 2 antibody
All lanes : Anti-PADI2 / PAD2 antibody (ab56075) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 76 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
The length of PADI2 is currently uncertain. According to Strausberg et al. (2002) [PMID: 12477932] it is 437aa in length (~45kDa). However, according to Ishigami et al. (2002) [PMID: 12392711] PADI2 is 665aa (~75kDa).
ab56075 has not yet been referenced specifically in any publications.