Anti-PADI3 / PAD3 antibody [EPR12165(B)] Recombinant (ab172959)

Product name

Anti-PADI3 / PAD3 antibody [EPR12165(B)]
See all PADI3 / PAD3 primary antibodies
Description

Rabbit monoclonal [EPR12165(B)] to PADI3 / PAD3
Host species

Rabbit
Tested applications

Suitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P
Species reactivity

Reacts with: Human
Does not react with: Mouse, Rat
Immunogen

Synthetic peptide within Human PADI3/ PAD3 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9ULW8
Positive control
- Human fetal muscle, skeletal muscle and skin lysates.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12165(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab172959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
IP		1/10 - 1/100.

Application notes

Is unsuitable for ICC or IHC-P.

Function

Catalyzes the deimination of arginine residues of proteins.
Tissue specificity

Hair follicles, and epidermis at very low levels.
Sequence similarities

Belongs to the protein arginine deiminase family.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession Q9ULW8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 51702 Human
- Omim: 606755 Human
- SwissProt: Q9ULW8 Human
- Unigene: 149195 Human
Alternative names
- PADI3 antibody
- PADI3_HUMAN antibody
- PDI3 antibody
- Peptidyl arginine deiminase, type III antibody
- Peptidylarginine deiminase III antibody
- Protein arginine deiminase type 3 antibody
- Protein arginine deiminase type III antibody
- Protein-arginine deiminase type III antibody
- Protein-arginine deiminase type-3 antibody
see all

Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)

All lanes : Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959) at 1/1000 dilution

Lane 1 : Human fetal muscle lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Human skin lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 75 kDa
Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)

Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate immunoprecipitated using ab172959 at 1/10 dilution.

Ü Basmanav FB et al. Mutations in Three Genes Encoding Proteins Involved in Hair Shaft Formation Cause Uncombable Hair Syndrome. Am J Hum Genet 99:1292-1304 (2016). Read more (PubMed: 27866708) »

Publishing research using ab172959? Please let us know so that we can cite the reference in this datasheet.

Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)