Recombinant Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12165(B)] to PADI3 / PAD3
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-PADI3 / PAD3 antibody [EPR12165(B)]
See all PADI3 / PAD3 primary antibodies -
Description
Rabbit monoclonal [EPR12165(B)] to PADI3 / PAD3 -
Host species
Rabbit -
Tested Applications & Species
Application Species IP HumanWB Human -
Immunogen
Synthetic peptide within Human PADI3/ PAD3 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9ULW8 -
Positive control
- Human fetal muscle, skeletal muscle and skin lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12165(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
|
IHC-P |
Human
|
IP |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
|
|
IP |
1/10 - 1/100.
|
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 75 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Catalyzes the deimination of arginine residues of proteins. -
Tissue specificity
Hair follicles, and epidermis at very low levels. -
Sequence similarities
Belongs to the protein arginine deiminase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 51702 Human
- Omim: 606755 Human
- SwissProt: Q9ULW8 Human
- Unigene: 149195 Human
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Alternative names
- PADI3 antibody
- PADI3_HUMAN antibody
- PDI3 antibody
see all
Images
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All lanes : Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959) at 1/1000 dilution
Lane 1 : Human fetal muscle lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 75 kDa -
Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate immunoprecipitated using ab172959 at 1/10 dilution.
Protocols
Datasheets and documents
References (3)
ab172959 has been referenced in 3 publications.
- Chang X et al. PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer. Cancer Biol Med 16:729-742 (2019). PubMed: 31908891
- Chai Z et al. PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer. Cancer Cell Int 19:277 (2019). PubMed: 31708688
- Ü Basmanav FB et al. Mutations in Three Genes Encoding Proteins Involved in Hair Shaft Formation Cause Uncombable Hair Syndrome. Am J Hum Genet 99:1292-1304 (2016). PubMed: 27866708