Recombinant Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)
![Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)](https://www.abcam.com/ps/products/172/ab172959/Images/ab172959-196001-anti-padi3-pad3-antibody-epr12165b-western-blot.jpg "Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12165(B)] to PADI3 / PAD3
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PADI3 / PAD3 antibody [EPR12165(B)]
See all PADI3 / PAD3 primary antibodies -
Description
Rabbit monoclonal [EPR12165(B)] to PADI3 / PAD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PADI3/ PAD3 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9ULW8 -
Positive control
- Human fetal muscle, skeletal muscle and skin lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12165(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
|
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| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 75 kDa. |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for ICC or IHC-P.
Target
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Function
Catalyzes the deimination of arginine residues of proteins. -
Tissue specificity
Hair follicles, and epidermis at very low levels. -
Sequence similarities
Belongs to the protein arginine deiminase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 51702 Human
- Omim: 606755 Human
- SwissProt: Q9ULW8 Human
- Unigene: 149195 Human
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Alternative names
- PADI3 antibody
- PADI3_HUMAN antibody
- PDI3 antibody
see all
Images
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Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)All lanes : Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959) at 1/1000 dilution
Lane 1 : Human fetal muscle lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 75 kDa -
![Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)](https://www.abcam.com/ps/products/172/ab172959/Images/ab172959-196004-anti-padi3-pad3-antibody-epr12165b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate immunoprecipitated using ab172959 at 1/10 dilution.
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![Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)](https://www.abcam.com/ps/products/172/ab172959/Images/ab172959-3-benefits-of-recombinant-antibodies.png)
Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab172959 has been referenced in 3 publications.
- Chang X et al. PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer. Cancer Biol Med 16:729-742 (2019). PubMed: 31908891
- Chai Z et al. PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer. Cancer Cell Int 19:277 (2019). PubMed: 31708688
- Ü Basmanav FB et al. Mutations in Three Genes Encoding Proteins Involved in Hair Shaft Formation Cause Uncombable Hair Syndrome. Am J Hum Genet 99:1292-1304 (2016). PubMed: 27866708
