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I ordered PAD4 antibody (ab50247). It says it reacts with human, but predicted to react with mice. I tried in on mice tissue by Western Blot, but it doesn't work very well, and there're a lot of unrelated bands there. The antibodies we previously ordered from your company are very good, and I'm wondering whether you guys could produce PAD4 antibody that react with mice better. Thank you very much!
Asked on May 10 2012
Thank you for contacting Abcam.
If you could provide me with the protocol that you are using, I would be happy to see if there are any protocol suggestions I can make that may help resolve some of the issues we are having.
Also we have a number of other PADI4 / PAD4 antibodies in our catalogue that may work for mouse but we have not tested them, therefore you could take advantage of our testing discount program:
I will pass your comments about getting an antibody that we guarantee to work with mice to the relevant team.
If there is anything else I can help you with, please let me know.
Answered on May 10 2012