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The product spec sheet says that this antibody has an application as Western blotting and predicted molecular weight is 74 kDa. However, when I used this antibody to detect human PAD4 in biological fluids by WB, actual observed band size was between 50 and 75 kDa (around 67 kDa).
Can I consider the band that I detected as human PAD4?
If so, What kind of modification reduce the molecular weight?
Asked on May 03 2013
The PAD4 protein is predicted to run around 74 kDa based on the molecular weight of the amino acid sequence, however full length PAD4 has been shown to migrate around 67 kDa in SDS-PAGE:
The exact cause of this has not been determined, but some proteins do not run at their predicted molecular weights due to structure and/or amino acid charge. Looking through our other antibodies to PAD4, they do recognize a band below 70 kDa. Based on this information, the band on your blot around 67 kDa is most likely the specific PAD4 band.
Answered on May 03 2013