Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab50332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution. 2-4 µl of the antibody immunoprecipitatesPADI4 from DMSO-treated HL60 cells|
|WB||1/1000 - 1/2000. Predicted molecular weight: 74 kDa.|
FunctionCatalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Tissue specificityExpressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
Involvement in diseaseGenetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
Sequence similaritiesBelongs to the protein arginine deiminase family.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
- Information by UniProt
- EC 126.96.36.199 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
All lanes : Anti-PADI4 / PAD4 antibody (ab50332) at 1/1000 dilution
Lane 1 : 293-T cells
Lane 2 : 293-T cells transfected with PADI4 cDNA
Lane 3 : HL60 cells
Lane 4 : HL60 cells induced with 1.25% DMSO for three days
All lanes : Goat Anti-Rabbit IgG
Predicted band size: 74 kDa
Observed band size: ~62 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Kosgodage US et al. Peptidylarginine Deiminases Post-Translationally Deiminate Prohibitin and Modulate Extracellular Vesicle Release and MicroRNAs in Glioblastoma Multiforme. Int J Mol Sci 20:N/A (2018). Read more (PubMed: 30597867) »
- Lewis HD et al. Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation. Nat Chem Biol 11:189-91 (2015). Read more (PubMed: 25622091) »