Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)

Overview

  • Product name
    Anti-PADI4 / PAD4 antibody [EPR20706]
    See all PADI4 / PAD4 primary antibodies
  • Description
    Rabbit monoclonal [EPR20706] to PADI4 / PAD4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Mouse PADI4/ PAD4 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Z183

  • Positive control
    • IHC-P: Mouse spleen tissue. WB: Mouse embryo lysate; ES-D3 whole cell lysate; HEK-293T transfected with mouse PADI4 / PAD4 expression vector containing a myc-His-tag®, whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
    • Tissue specificity
      Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
    • Involvement in disease
      Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
    • Sequence similarities
      Belongs to the protein arginine deiminase family.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 3.5.3.15 antibody
      • HL 60 PAD antibody
      • HL-60 PAD antibody
      • PAD 4 antibody
      • PAD antibody
      • PADI 4 antibody
      • PADI 5 antibody
      • PADI H protein antibody
      • Padi4 antibody
      • PADI4_HUMAN antibody
      • PADI5 antibody
      • PDI 4 antibody
      • PDI 5 antibody
      • PDI4 antibody
      • PDI5 antibody
      • Peptidyl arginine deiminase type IV antibody
      • Peptidyl arginine deiminase type V antibody
      • Peptidylarginine deiminase IV antibody
      • Protein arginine deiminase antibody
      • Protein arginine deiminase type 4 antibody
      • Protein arginine deiminase type IV antibody
      • Protein-arginine deiminase type IV antibody
      • Protein-arginine deiminase type-4 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID: 20733033, PMID: 28281906, PMID: 12393868, PMID: 12833157). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution

      Lane 1 : Wild-type mouse embryonic stem cells (E14) with BSA
      Lanes 2-3 : mouse embryonic stem cells (E14) PADI4 Knock out with BSA

      Lysates/proteins at 40 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under non-reducing conditions.

      Predicted band size: 74 kDa


      Exposure time: 18 hours

      See Abreview

    • All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T transfected with mouse PADI4 / PAD4 expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 74 kDa
      Observed band size: 74 kDa


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution

      Lane 1 : Mouse embryo lysate
      Lane 2 : ES-D3 (mouse embryonic multipotent stem cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 74 kDa
      Observed band size: 74 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:
    • Perdomo J  et al. Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia. Nat Commun 10:1322 (2019). WB ; Mouse . Read more (PubMed: 30899022) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells (E14))
    Gel Running Conditions
    Non-reduced Denaturing (4-20% gradient gel)
    Loading amount
    40 µg
    Specification
    mouse embryonic stem cells (E14)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Maria Christophorou

    Verified customer

    Submitted Jan 09 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up