Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
  • Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20706] to PADI4 / PAD4 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free
    See all PADI4 / PAD4 primary antibodies

  • Description

    Rabbit monoclonal [EPR20706] to PADI4 / PAD4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen and lung tissues. Rat lung tissue.

  • General notes

    Ab227891 is the carrier-free version of ab214810. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227891 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

  • Tissue specificity

    Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.

  • Involvement in disease

    Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.

  • Sequence similarities

    Belongs to the protein arginine deiminase family.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 3.5.3.15 antibody
      • HL 60 PAD antibody
      • HL-60 PAD antibody
      • PAD 4 antibody
      • PAD antibody
      • PADI 4 antibody
      • PADI 5 antibody
      • PADI H protein antibody
      • Padi4 antibody
      • PADI4_HUMAN antibody
      • PADI5 antibody
      • PDI 4 antibody
      • PDI 5 antibody
      • PDI4 antibody
      • PDI5 antibody
      • Peptidyl arginine deiminase type IV antibody
      • Peptidyl arginine deiminase type V antibody
      • Peptidylarginine deiminase IV antibody
      • Protein arginine deiminase antibody
      • Protein arginine deiminase type 4 antibody
      • Protein arginine deiminase type IV antibody
      • Protein-arginine deiminase type IV antibody
      • Protein-arginine deiminase type-4 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)

      This data was developed using ab214810, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in rat lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)

      This data was developed using ab214810, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in mouse spleen.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)

      This data was developed using ab214810, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in mouse lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID: 20733033, PMID: 28281906, PMID: 12393868, PMID: 12833157). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214810).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
      Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab227891? Please let us know so that we can cite the reference in this datasheet.

    ab227891 has not yet been referenced specifically in any publications.

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