Recombinant Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
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Overview
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Product nameAnti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free
See all PADI4 / PAD4 primary antibodies -
DescriptionRabbit monoclonal [EPR20706] to PADI4 / PAD4 - BSA and Azide free
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse
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Immunogen
Synthetic peptide within Mouse PADI4/ PAD4 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Z183 -
Positive control
- IHC-P: Mouse spleen tissue.
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General notes
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
ab227891 is a PBS-only buffer format of ab214810. Please refer to ab214810 for recommended dilutions, protocols, and image data.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR20706
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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FunctionCatalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
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Tissue specificityExpressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
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Involvement in diseaseGenetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
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Sequence similaritiesBelongs to the protein arginine deiminase family.
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Cellular localizationCytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
- Information by UniProt
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Database links
- Entrez Gene: 18602 Mouse
- SwissProt: Q9Z183 Mouse
- Unigene: 250358 Mouse
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Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)](https://www.abcam.com/ps/products/227/ab227891/Images/ab227891-291226-21481011.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID: 20733033, PMID: 28281906, PMID: 12393868, PMID: 12833157). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214810).
Datasheets and documents
References
ab227891 has not yet been referenced specifically in any publications.
