Recombinant Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free
See all PADI4 / PAD4 primary antibodies -
Description
Rabbit monoclonal [EPR20706] to PADI4 / PAD4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse PADI4/ PAD4 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Z183 -
Positive control
- IHC-P: Mouse spleen tissue.
-
General notes
Ab227891 is the carrier-free version of ab214810. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab227891 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20706 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab227891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. -
Tissue specificity
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. -
Involvement in disease
Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. -
Sequence similarities
Belongs to the protein arginine deiminase family. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils. - Information by UniProt
-
Database links
- Entrez Gene: 18602 Mouse
- SwissProt: Q9Z183 Mouse
- Unigene: 250358 Mouse
-
Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)](https://www.abcam.com/ps/products/227/ab227891/Images/ab227891-291226-21481011.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID: 20733033, PMID: 28281906, PMID: 12393868, PMID: 12833157). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214810).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
Certificate of Compliance
References
ab227891 has not yet been referenced specifically in any publications.
