Name: Anti-PADI4 / PAD4 antibody [OTI4H5]
SKU: ab128086
Rating: 5 (1 reviews)

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Secondary

Protein

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Primary - Mouse Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
WB, IHC-P, Flow Cyt, ICC/IF

Secondary - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
WB, IP, ELISA, IHC-P

Protein - Recombinant Human PADI4 / PAD4 protein (ab126910)
SDS-PAGE, WB

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Product name

Anti-PADI4 / PAD4 antibody [OTI4H5]
See all PADI4 / PAD4 primary antibodies
Description

Mouse monoclonal [OTI4H5] to PADI4 / PAD4
Host species

Mouse
Tested applications

Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein, corresponding to amino acids 1-663 of Human PADI4/ PAD4 produced in HEK293T cells (NP_036519).
Positive control
- HEK293T cells transfected with PADI4 / PAD4 , Human breast, Carcinoma of Human lung, Adenocarcinoma of Human ovary, Human pancreas, Adenocarcinoma of Human endometrium, Human prostate, Carcinoma of Human prostate, Carcinoma of Human bladder and Human lymphoma tissues, COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 , Hela and Jurkat cells
General notes

Dilute in PBS (pH7.3) before use.

The clone number has been updated from 4H5 to OTI4H5, both clone numbers name the same antibody clone.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS
Clonality

Monoclonal
Clone number

OTI4H5
Isotype

IgG2a
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
Recombinant Protein
- Recombinant Human PADI4 / PAD4 protein (ab126910)

Our Abpromise guarantee covers the use of ab128086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/2000. Predicted molecular weight: 74 kDa.
IHC-P		1/150.
Flow Cyt		1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF		1/100.

Function

Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Tissue specificity

Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
Involvement in disease

Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
Sequence similarities

Belongs to the protein arginine deiminase family.
Cellular localization

Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
Target information above from: UniProt accession Q9UM07 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 23569 Human
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
- PAD 4 antibody
- PAD antibody
- PADI 4 antibody
- PADI 5 antibody
- PADI H protein antibody
- Padi4 antibody
- PADI4_HUMAN antibody
- PADI5 antibody
- PDI 4 antibody
- PDI 5 antibody
- PDI4 antibody
- PDI5 antibody
- Peptidyl arginine deiminase type IV antibody
- Peptidyl arginine deiminase type V antibody
- Peptidylarginine deiminase IV antibody
- Protein arginine deiminase antibody
- Protein arginine deiminase type 4 antibody
- Protein arginine deiminase type IV antibody
- Protein-arginine deiminase type IV antibody
- Protein-arginine deiminase type-4 antibody
see all

Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

All lanes : Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) at 1/2000 dilution

Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PADI4 / PAD4 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 74 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206501)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/100, staining COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 by Immunofluorescence.
Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in HeLa cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometric.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded carcinoma of Human lung tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human ovary tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human prostate tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human bladder tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in Jurkat cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometric.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Liu M et al. PADI4-mediated epithelial-mesenchymal transition in lung cancer cells. Mol Med Rep 19:3087-3094 (2019). Read more (PubMed: 30816464) »
Liu C et al. PADI4 stimulates esophageal squamous cell carcinoma tumor growth and up-regulates CA9 expression. Mol Carcinog 58:66-75 (2019). Read more (PubMed: 30242913) »

See all 19 Publications for this product

Publishing research using ab128086? Please let us know so that we can cite the reference in this datasheet.

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1-2 of 2 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Human Kidney Tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate based (Vector Laboratories H-3300)

Permeabilization

Yes - 0.2% Triton-X 10 mins RT

Specification

Human Kidney Tissue

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Jan 29 2018

Question

Hello,
I would like to order a reliable pad4 antibody for immunofluorescence. Can you recommend, please, which of the antibodies ab128086 or ab50247 is the best for this application? Also, it is important for me to get the exact concentration of the antibody I'm going to use, please.
Many thanks.

Abcam community

Verified customer

Asked on Jan 18 2013

Answer

Thank you for contacting us.

The exact concentration is necessary when you would like to use Isotype control in IHC or Immunoprecipitation other than that it is OK.

Please note that we can only provide exact concentration for ab128089 because it is purified antibody. We cannot provide concentration for ab50247 because it is a un-purified antibody.

I can recommend buying ab128086.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Jan 18 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PADI4 / PAD4 antibody [OTI4H5]