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Signal Transduction Metabolism Amino Acids
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Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

  • Datasheet
Reviews (1)Q&A (1)References (21)

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Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
  • Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)

Key features and details

  • Mouse monoclonal [OTI4H5] to PADI4 / PAD4
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG2a

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View more associated products

Overview

  • Product name

    Anti-PADI4 / PAD4 antibody [OTI4H5]
    See all PADI4 / PAD4 primary antibodies
  • Description

    Mouse monoclonal [OTI4H5] to PADI4 / PAD4
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-663 of Human PADI4/ PAD4 produced in HEK293T cells (NP_036519).

  • Positive control

    • HEK293T cells transfected with PADI4 / PAD4 , Human breast, Carcinoma of Human lung, Adenocarcinoma of Human ovary, Human pancreas, Adenocarcinoma of Human endometrium, Human prostate, Carcinoma of Human prostate, Carcinoma of Human bladder and Human lymphoma tissues, COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 , Hela and Jurkat cells
  • General notes

    The clone number has been updated from 4H5 to OTI4H5, both clone numbers name the same antibody clone.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    OTI4H5
  • Isotype

    IgG2a
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Arginine Deiminases
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Arginine Deimination
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human PADI4 / PAD4 protein (ab126910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
African green monkey
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/2000. Predicted molecular weight: 74 kDa.
IHC-P (1)
1/150.
Flow Cyt
1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF
1/100.
Notes
WB
1/2000. Predicted molecular weight: 74 kDa.
IHC-P
1/150.
Flow Cyt
1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF
1/100.

Target

  • Function

    Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • Tissue specificity

    Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
  • Involvement in disease

    Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
  • Sequence similarities

    Belongs to the protein arginine deiminase family.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
  • Target information above from: UniProt accession Q9UM07 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23569 Human
    • Omim: 605347 Human
    • SwissProt: Q9UM07 Human
    • Unigene: 522969 Human
    • Alternative names

      • EC 3.5.3.15 antibody
      • HL 60 PAD antibody
      • HL-60 PAD antibody
      • PAD 4 antibody
      • PAD antibody
      • PADI 4 antibody
      • PADI 5 antibody
      • PADI H protein antibody
      • Padi4 antibody
      • PADI4_HUMAN antibody
      • PADI5 antibody
      • PDI 4 antibody
      • PDI 5 antibody
      • PDI4 antibody
      • PDI5 antibody
      • Peptidyl arginine deiminase type IV antibody
      • Peptidyl arginine deiminase type V antibody
      • Peptidylarginine deiminase IV antibody
      • Protein arginine deiminase antibody
      • Protein arginine deiminase type 4 antibody
      • Protein arginine deiminase type IV antibody
      • Protein-arginine deiminase type IV antibody
      • Protein-arginine deiminase type-4 antibody
      see all

    Images

    • Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      All lanes : Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) at 1/2000 dilution

      Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
      Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PADI4 / PAD4 cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 74 kDa



      HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206501)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human breast tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/100, staining COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 by Immunofluorescence.
    • Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in HeLa cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometric.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded carcinoma of Human lung tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human ovary tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human prostate tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human bladder tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
    • Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
      ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in Jurkat cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometric.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (21)

    Publishing research using ab128086? Please let us know so that we can cite the reference in this datasheet.

    ab128086 has been referenced in 21 publications.

    • de Bont CM  et al. Neutrophil proteases degrade autoepitopes of NET-associated proteins. Clin Exp Immunol 199:1-8 (2020). PubMed: 31661552
    • Casanova V  et al. Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection. Front Immunol 11:85 (2020). PubMed: 32117246
    • Liu C  et al. PADI4 stimulates esophageal squamous cell carcinoma tumor growth and up-regulates CA9 expression. Mol Carcinog 58:66-75 (2019). PubMed: 30242913
    • Liu M  et al. PADI4-mediated epithelial-mesenchymal transition in lung cancer cells. Mol Med Rep 19:3087-3094 (2019). PubMed: 30816464
    • Wolach O  et al. Increased neutrophil extracellular trap formation promotes thrombosis in myeloproliferative neoplasms. Sci Transl Med 10:N/A (2018). PubMed: 29643232
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PADI4 / PAD4 antibody [OTI4H5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Kidney Tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate based (Vector Laboratories H-3300)
    Permeabilization
    Yes - 0.2% Triton-X 10 mins RT
    Specification
    Human Kidney Tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 29 2018

    Question

    Hello,
    I would like to order a reliable pad4 antibody for immunofluorescence. Can you recommend, please, which of the antibodies ab128086 or ab50247 is the best for this application? Also, it is important for me to get the exact concentration of the antibody I'm going to use, please.
    Many thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 18 2013

    Answer

    Thank you for contacting us.

    The exact concentration is necessary when you would like to use Isotype control in IHC or Immunoprecipitation other than that it is OK.

    Please note that we can only provide exact concentration for ab128089 because it is purified antibody. We cannot provide concentration for ab50247 because it is a un-purified antibody.

    I can recommend buying ab128086.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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