• Product name
    Anti-PADI4 / PAD4 antibody
    See all PADI4 / PAD4 primary antibodies
  • Description
    Goat polyclonal to PADI4 / PAD4
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 2-14 of Human PADI4/ PAD4

  • Positive control
    • Human spleen and human liver lysates for Western blot. Tansfected HEK293 transiently expressing PADI4 / PAD4.



Our Abpromise guarantee covers the use of ab26071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with Human PADI4 / PAD4 peptide (ab30475).
IHC-P Use a concentration of 5 µg/ml.


  • Function
    Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • Tissue specificity
    Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
  • Involvement in disease
    Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
  • Sequence similarities
    Belongs to the protein arginine deiminase family.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC antibody
    • HL 60 PAD antibody
    • HL-60 PAD antibody
    • PAD 4 antibody
    • PAD antibody
    • PADI 4 antibody
    • PADI 5 antibody
    • PADI H protein antibody
    • Padi4 antibody
    • PADI4_HUMAN antibody
    • PADI5 antibody
    • PDI 4 antibody
    • PDI 5 antibody
    • PDI4 antibody
    • PDI5 antibody
    • Peptidyl arginine deiminase type IV antibody
    • Peptidyl arginine deiminase type V antibody
    • Peptidylarginine deiminase IV antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 4 antibody
    • Protein arginine deiminase type IV antibody
    • Protein-arginine deiminase type IV antibody
    • Protein-arginine deiminase type-4 antibody
    see all


  • Immunohistochemical analysis of paraffin embedded Human spleen tissue staining PADI4/PAD4 using ab26071 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP staining.
  • Anti-PADI4 / PAD4 antibody (ab26071) at 0.3 µg/ml + human spleen lysate (35µg protein in RIPA buffer).

    Developed using the ECL technique.

    Predicted band size: 74 kDa
    Observed band size: 74 kDa

    Primary incubation was 1 hour.
  • Anti-PADI4 / PAD4 antibody (ab26071) at 1 µg/ml + transfected HEK293 transiently expressing PADI4 / PAD4

    Predicted band size: 74 kDa
    Observed band size: 74 kDa


This product has been referenced in:
  • Kinloch A  et al. Synovial fluid is a site of citrullination of autoantigens in inflammatory arthritis. Arthritis Rheum 58:2287-95 (2008). WB ; Human . Read more (PubMed: 18668562) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for getting back to me. The catalogue code for our PADI4 / PAD4 peptide (2-14) is ab30475. The price in USD is 150 for 100ug.

Thank you for your enquiry. A band at approximately 74kDa was observed in Human Spleen and Human Liver lysates (calculated MW of 74.1kDa according to NP_036519). Recommended concentration: 0.3-2µg/ml. Below please find additional protocol detail...

Read More


Sign up