Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab16480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    IF/ICC: see image below

    This antibody gave a positive result in ELISA against the immunizing peptide (ab17094).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalyzes the deimination of arginine residues of proteins. May be involved in cytoskeletal reorganization in the egg and early embryo.
    • Tissue specificity
      Ovary, testis and peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the protein arginine deiminase family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • hPADVI antibody
      • PADI6 antibody
      • PADI6_HUMAN antibody
      • peptidyl arginine deiminase like protein antibody
      • peptidyl arginine deiminase type VI antibody
      • Peptidylarginine deiminase type 6 antibody
      • Peptidylarginine deiminase type VI antibody
      • Peptidylarginine deiminase VI antibody
      • protein arginine deiminase type 6 antibody
      • Protein arginine deiminase type VI antibody
      • Protein-arginine deiminase type VI antibody
      • Protein-arginine deiminase type-6 antibody
      see all

    Images

    • All lanes : Anti-PADI6 antibody (ab16480) at 1 µg/ml

      Lane 1 : Human ovary lysate

      Lane 2 : Human testes lysate

      Lane 3 : Human ovary lysate
      with Human PADI6 peptide (ab17094) at 1 µg/ml
      Lane 4 : Human testes lysate
      with Human PADI6 peptide (ab17094) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

      Developed using the ECL technique.


      The predicted band at 77kD was not detected in these lysates. This could be attributed to low expression levels of PADI6 in these lysates.
    • ICC/IF image of ab16480 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16480, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 100% methanol fixed cells at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

    References

    This product has been referenced in:
    • Xu Y  et al. Mutations in PADI6 Cause Female Infertility Characterized by Early Embryonic Arrest. Am J Hum Genet 99:744-752 (2016). Read more (PubMed: 27545678) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa #Jurkat #HL-60)
    Loading amount
    30 µg
    Specification
    HeLa #Jurkat #HL-60
    Gel Running Conditions
    Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Mr. Antibody Solutions

    Verified customer

    Submitted Oct 28 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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