Overview

  • Product name

    Anti-PAEP/Glycodelin antibody
    See all PAEP/Glycodelin primary antibodies
  • Description

    Rabbit polyclonal to PAEP/Glycodelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PAEP/Glycodelin aa 72-101 (internal sequence) conjugated to keyhole limpet haemocyanin. (NP_001018059.1, NP_002562.2)

  • Positive control

    • WiDr cell line lysate.
  • General notes

    Previously labelled as Placental Protein 14 / Glycodelin A. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.

Target

  • Function

    This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Post-translational
    modifications

    At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha uterine protein antibody
    • gD antibody
    • GdA antibody
    • GdF antibody
    • GdS antibody
    • Glycodelin A antibody
    • Glycodelin antibody
    • Glycodelin F antibody
    • Glycodelin S antibody
    • MGC138509 antibody
    • MGC142288 antibody
    • PAEG antibody
    • PAEP antibody
    • PAEP_HUMAN antibody
    • PEG antibody
    • PEP antibody
    • Placental Protein 14 / Glycodelin A antibody
    • Placental protein 14 antibody
    • PP14 antibody
    • Pregnancy-associated endometrial alpha-2 globulin antibody
    • Progestagen-associated endometrial protein antibody
    • Progesterone-associated endometrial protein antibody
    see all

Images

  • Anti-PAEP/Glycodelin antibody (ab123318) at 1/100 dilution + WiDr cell line lysates at 35 µg

    Developed using the ECL technique.

    Predicted band size: 18 kDa



    ab123318 is recognizing isoform 2 of Human PAEP / Glycodelin.

References

ab123318 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (OV90)
Permeabilization
Yes - 0.25% Triton-X 100 in PBS
Specification
OV90
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA231 human tumor from Balb/C nude mice)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
MDA231 human tumor from Balb/C nude mice
Blocking step
innovex background buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 05 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (OV90 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine)
Loading amount
50000 cells
Specification
OV90 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 31 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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