Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP870Y] to PAEP/Glycodelin
- Suitable for: WB
- Reacts with: Human
Product nameAnti-PAEP/Glycodelin antibody [EP870Y]
See all PAEP/Glycodelin primary antibodies
DescriptionRabbit monoclonal [EP870Y] to PAEP/Glycodelin
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human PAEP/Glycodelin aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: P09466
- WB: TPA-treated K562 cell lysate. Human placenta, kidney, and brain tissue lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Placental Protein 14 / Glycodelin A.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab53289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
FunctionThis protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy.
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
modificationsAt least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction.
- Information by UniProt
- Alpha uterine protein antibody
- gD antibody
- GdA antibody
All lanes : Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289) at 0.3 µg/ml (purified)
Lane 1 : Human placenta lysates
Lane 2 : Human kidney lysates
Lane 3 : Human brain lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289) at 1/500 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : TPA-treated K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab53289 has been referenced in 2 publications.
- Turco MY et al. Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium. Nat Cell Biol 19:568-577 (2017). WB ; Human . PubMed: 28394884
- Uysal S et al. Correlation of endometrial glycodelin expression and pregnancy outcome in cases with polycystic ovary syndrome treated with clomiphene citrate plus metformin: a controlled study. Obstet Gynecol Int 2015:278591 (2015). PubMed: 25815012