Overview

  • Product name

  • Description

    Goat polyclonal to PAF-1
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human PAF-1 aa 73-87 (internal sequence).
    Sequence:

    C-NATVGQSVLNIKYKN

  • Positive control

    • HeLa lysate
  • General notes

    Protein previously labeled as PEX2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab101722 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Somewhat implicated in the biogenesis of peroxisomes.
  • Involvement in disease

    Defects in PEX2 are the cause of peroxisome biogenesis disorder complementation group 5 (PBD-CG5) [MIM:170993]; also known as PBD-CGF. PBD refers to a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix. The PBD group is comprised of four disorders: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum. The PBD group is genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies.
    Defects in PEX2 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life.
    Defects in PEX2 are a cause of infantile Refsum disease (IRD) [MIM:266510]. IRD is a mild peroxisome biogenesis disorder (PBD). Clinical features include early onset, mental retardation, minor facial dysmorphism, retinopathy, sensorineural hearing deficit, hepatomegaly, osteoporosis, failure to thrive, and hypocholesterolemia. The biochemical abnormalities include accumulation of phytanic acid, very long chain fatty acids (VLCFA), di- and trihydroxycholestanoic acid and pipecolic acid.
  • Sequence similarities

    Belongs to the pex2/pex10/pex12 family.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Peroxisome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 35 kDa peroxisomal membrane protein antibody
    • PAF-1 antibody
    • PAF1 antibody
    • Peroxin-2 antibody
    • peroxisomal biogenesis factor 2 antibody
    • Peroxisomal membrane protein 3 antibody
    • peroxisomal membrane protein 3, 35kDa antibody
    • Peroxisome assembly factor 1 antibody
    • Peroxisome biogenesis factor 2 antibody
    • PEX2 antibody
    • PEX2_HUMAN antibody
    • PMP3 antibody
    • PMP35 antibody
    • PXMP3 antibody
    • RING finger protein 72 antibody
    • RNF72 antibody
    • ZWS3 antibody
    see all

Images

  • Anti-PAF-1 antibody (ab101722) at 0.5 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

References

ab101722 has not yet been referenced specifically in any publications.

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