Overview

  • Product name

    PAF Acetylhydrolase Assay Kit
    See all PAF Acetylhydrolase kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    PAF-AH 89 3.5%
    Inter-assay
    Sample n Mean SD CV%
    PAF-AH 89 10%
  • Sample type

    Serum, Plasma, Cell Lysate, Tissue Homogenate
  • Assay type

    Enzyme activity
  • Range

    0.02 µmol/min/ml - 0.2 µmol/min/ml
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Abcam's PAF Acetylhydrolase Assay Kit (ab133088) provides an accurate and convenient method for measurement of PAF acetylhydrolase (PAF-AH) activity (both cytosolic and extracellular). The assay uses 2-thio PAF which serves as a substrate for all PAF-AHs. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5’-dithio-bis-(2- nitrobenzoic acid).

  • Notes

    Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). PAF-AHs are located intra- and extra-cellularly (for instance, in both cytosol and plasma)  Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.

  • Platform

    Microplate reader

Properties

Images

Protocols

References

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