Overview

  • Product name

    PAF Acetylhydrolase Inhibitor Screening Assay Kit
    See all PAF Acetylhydrolase kits
  • Detection method

    Colorimetric
  • Assay type

    Enzyme activity
  • Product overview

    ab133091 uses 2-thio PAF as a substrate for PAF Acetylhydrolase (PAF-AH). Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF Acetylhydrolase, free thiols are detected using 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB). The PAF Acetylhydrolase Inhibitor Screening assay includes Human plasma PAF Acetylhydrolase as control and is a time saving tool for screening vast numbers of inhibitors.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 96 tests
    2-thio PAF (substrate) 2 vials
    96-Well Plate (Colorimetric Assay) 1 unit
    96-Well Plate Cover 1 unit
    Human Plasma PAF-AH 2 vials
    PAF-AH Assay Buffer (10X) 1 vial
    PAF-AH Assay DTNB 4 vials
  • Research areas

  • Function

    Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.
  • Sequence similarities

    In the N-terminal section; belongs to the asparaginase 1 family.
    Contains 5 ANK repeats.
  • Information by UniProt
  • Alternative names

    • 60 kDa lysophospholipase
    • A530050D06Rik
    • AI429460
    • asparaginase homolog (S. cerevisiae)
    • ASPG
    • C14orf76
    • Gm690
    • GPA/WT
    • L-asparaginase
    • L-asparagine amidohydrolase
    • LPP60_HUMAN
    • PAF acetylhydrolase
    • Platelet-activating factor acetylhydrolase
    • Wild type L asparaginase
    see all

Images

  • Inhibition of Human plasma PAF Acetylhydrolase by MAFP (IC50 = 250 nM).

Protocols

References

ab133091 has not yet been referenced specifically in any publications.

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