Overview

  • Product name

  • Description

    Rabbit polyclonal to PAF-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human PAF-R aa 1-17 (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    MEPHDSSHMDSEFRYTL


    (Peptide available as ab107706)

  • Positive control

    • A549 membrane fraction. U937 and Raji cell lysates.
  • General notes

    Previously labelled as PAF Receptor. 

Properties

Applications

Our Abpromise guarantee covers the use of ab104162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 39 kDa.Can be blocked with PAF-R peptide (ab107706).
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-PAF-R antibody (ab104162) at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : A549 membrane fraction at 30 µg

    Secondary
    All lanes : Anti-rabbit IgG (HRP) conjugate at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa

  • PAF-R was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to PAF-R and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104162.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 39kDa; PAF-R

References

This product has been referenced in:

  • Liang H  et al. LncRNA PFL contributes to cardiac fibrosis by acting as a competing endogenous RNA of let-7d. Theranostics 8:1180-1194 (2018). Read more (PubMed: 29464008) »
  • Patel PS & Kearney JF CD36 and Platelet-Activating Factor Receptor Promote House Dust Mite Allergy Development. J Immunol 199:1184-1195 (2017). Read more (PubMed: 28667161) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Blocking
Sample
Streptococcus pneumoniae Cell (Primary Bronchial Epithelial cells)
Specification
Primary Bronchial Epithelial cells

Dr. Selvarani Vimalanathan

Verified customer

Submitted Aug 24 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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