Product nameAnti-PAF-R antibody
See all PAF-R primary antibodies
DescriptionRabbit polyclonal to PAF-R
Tested applicationsSuitable for: WB, ICC, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig, Rhesus monkey
- A549 membrane fraction. U937 and Raji cell lysates.
Previously labelled as PAF Receptor.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.05% BSA, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab104162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Predicted molecular weight: 39 kDa.Can be blocked with PAF-R peptide (ab107706).|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
FunctionReceptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system.
Tissue specificityExpressed in the placenta, lung, left and right heart ventricles, heart atrium, leukocytes and differentiated HL-60 granulocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- OTTHUMP00000004041 antibody
- OTTMUSP00000010350 antibody
- PAF R antibody
All lanes : Anti-PAF-R antibody (ab104162) at 1 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : A549 membrane fraction at 30 µg
All lanes : Anti-rabbit IgG (HRP) conjugate at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
PAF-R was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to PAF-R and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104162.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 39kDa; PAF-R
This product has been referenced in:
- Liang H et al. LncRNA PFL contributes to cardiac fibrosis by acting as a competing endogenous RNA of let-7d. Theranostics 8:1180-1194 (2018). Read more (PubMed: 29464008) »
- Patel PS & Kearney JF CD36 and Platelet-Activating Factor Receptor Promote House Dust Mite Allergy Development. J Immunol 199:1184-1195 (2017). Read more (PubMed: 28667161) »