Overview

  • Product name

  • Description

    Rabbit polyclonal to PAF1/PD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human PAF1/PD2 aa 232-481.

  • Positive control

    • Cal27 xenograft tissue, 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji cell lysates. This antibody gave a positive result when used in the following methanol fixed cell lines: T47D
  • General notes

    Previously labelled as PAF1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab137519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

Images

  • All lanes : Anti-PAF1/PD2 antibody (ab137519) at 1/5000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 60 kDa



    7.5 % SDS PAGE
  • ICC/IF image of ab137519 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab137519 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labelling PAF1/PD2 using ab137519 at 1/500.

References

This product has been referenced in:

  • Zhang M  et al. A peptide encoded by circular form of LINC-PINT suppresses oncogenic transcriptional elongation in glioblastoma. Nat Commun 9:4475 (2018). Read more (PubMed: 30367041) »
  • Herr P  et al. A genome-wide IR-induced RAD51 foci RNAi screen identifies CDC73 involved in chromatin remodeling for DNA repair. Cell Discov 1:15034 (2015). Human . Read more (PubMed: 27462432) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293T)
Total protein in input
1.27 µg
Immuno-precipitation step
Protein G
Specification
HEK 293T

Abcam user community

Verified customer

Submitted Nov 17 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up