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  1. Link

    paf1pd2-antibody-ab137519.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription Polymerase
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Validated using a knockout cell line

Anti-PAF1/PD2 antibody (ab137519)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Western blot - Anti-PAF1/PD2 antibody (ab137519)
  • Western blot - Anti-PAF1/PD2 antibody (ab137519)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAF1/PD2 antibody (ab137519)

Key features and details

  • Rabbit polyclonal to PAF1/PD2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PAF1/PD2 antibody
    See all PAF1/PD2 primary antibodies
  • Description

    Rabbit polyclonal to PAF1/PD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human PAF1/PD2 aa 232-481.

  • Positive control

    • WB: Wild-type HEK-293T, A549, Daudi, A431 and H1299 whole cell lysates. IHC-P: Cal27 xenograft tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Polymerase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Elongation factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    The mammalian PAF1 complex is a multifunctional complex. The PAF1 complex interacts with RNA polymerase II and may be involved in both initiation and elongation, and in histone methylation and RNA processing. Has oncogenic activity in vivo and in vitro.
  • Sequence similarities

    Belongs to the PAF1 family.
  • Cellular localization

    Nucleus. Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin.
  • Target information above from: UniProt accession Q8N7H5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 528744 Cow
    • Entrez Gene: 54623 Human
    • Entrez Gene: 54624 Mouse
    • Entrez Gene: 361531 Rat
    • Omim: 610506 Human
    • SwissProt: Q2KJ14 Cow
    • SwissProt: Q8N7H5 Human
    • SwissProt: Q8K2T8 Mouse
    • SwissProt: Q4V886 Rat
    • Unigene: 466714 Human
    • Unigene: 7916 Mouse
    • Unigene: 161558 Rat
    see all
  • Alternative names

    • F23149 1 antibody
    • FLJ11123 antibody
    • hPAF1 antibody
    • PAF 1 antibody
    • PAF1 antibody
    • Paf1 RNA polymerase II associated factor homolog antibody
    • PAF1_HUMAN antibody
    • Pancreatic differentiation protein 2 antibody
    • PD 2 antibody
    • PD2 antibody
    • PD2 protein antibody
    • RNA polymerase II associated factor homolog antibody
    • RNA polymerase II-associated factor 1 homolog antibody
    see all

Images

  • Western blot - Anti-PAF1/PD2 antibody (ab137519)
    Western blot - Anti-PAF1/PD2 antibody (ab137519)
    All lanes : Anti-PAF1/PD2 antibody (ab137519) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PAF1 CRISPR-Cas9 Edited HEK-293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab137519 observed at 80 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab137519 was shown to react with PAF1/PD2 in wild-type HEK-293T cells in Western blot with loss of signal observed at the expected molecular weight in PAF1 CRISPR-Cas9 edited cell line. The band observed at the higher molecular weight in the CRISPR-Cas9 edited sample is likely to represent PAF1 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. Wild-type HEK-293T and PAF1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab137519 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-PAF1/PD2 antibody (ab137519)
    Western blot - Anti-PAF1/PD2 antibody (ab137519)
    All lanes : Anti-PAF1/PD2 antibody (ab137519) at 1/5000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 60 kDa



    7.5 % SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAF1/PD2 antibody (ab137519)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAF1/PD2 antibody (ab137519)

    Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labelling PAF1/PD2 using ab137519 at 1/500.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab137519? Please let us know so that we can cite the reference in this datasheet.

ab137519 has been referenced in 4 publications.

  • Zheng JJ  et al. Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription. Radiat Oncol 15:131 (2020). PubMed: 32471508
  • Zhang M  et al. A peptide encoded by circular form of LINC-PINT suppresses oncogenic transcriptional elongation in glioblastoma. Nat Commun 9:4475 (2018). PubMed: 30367041
  • Herr P  et al. A genome-wide IR-induced RAD51 foci RNAi screen identifies CDC73 involved in chromatin remodeling for DNA repair. Cell Discov 1:15034 (2015). Human . PubMed: 27462432
  • Chen FX  et al. PAF1, a Molecular Regulator of Promoter-Proximal Pausing by RNA Polymerase II. Cell 162:1003-15 (2015). PubMed: 26279188

Customer reviews and Q&As

Show All Reviews Q&A
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Immunoprecipitation abreview for Anti-PAF1/PD2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293T)
Total protein in input
1.27 µg
Immuno-precipitation step
Protein G
Specification
HEK 293T
Read More

Abcam user community

Verified customer

Submitted Nov 17 2017

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