Recombinant
RabMAb

Anti-PAFAH1B2 antibody [EPR11250] (ab157479)

Submit an Abreview Submit a Question

Overview

  • Product name
    Anti-PAFAH1B2 antibody [EPR11250]
  • Description
    Rabbit monoclonal [EPR11250] to PAFAH1B2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human PAFAH1B2 (UniProt P68402).

  • Positive control
    • SH-SY5Y, 293T, Human testis and K562 lysates; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function
      Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Intracellular platelet activating factor acetylhydrolase alpha 2 subunit antibody
      • PA1B2_HUMAN antibody
      • PAF acetylhydrolase 30 kDa subunit antibody
      • PAF AH 30 kDa subunit antibody
      • PAF AH beta subunit antibody
      • PAF AH subunit beta antibody
      • PAF-AH 30 kDa subunit antibody
      • PAF-AH subunit beta antibody
      • PAFAH 30 kDa subunit antibody
      • PAFAH beta subunit antibody
      • PAFAH subunit beta antibody
      • Pafah1b2 antibody
      • PAFAH1B2 protein antibody
      • PAFAHB antibody
      • Platelet activating factor acetylhydrolase 1b catalytic subunit 2 antibody
      • Platelet activating factor acetylhydrolase IB beta subunit antibody
      • Platelet activating factor acetylhydrolase IB subunit beta antibody
      • Platelet activating factor acetylhydrolase isoform Ib beta subunit antibody
      • Platelet activating factor acetylhydrolase isoform Ib subunit 2 antibody
      • Platelet-activating factor acetylhydrolase IB subunit beta antibody
      see all

    Images

    • Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
      Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
      All lanes : Anti-PAFAH1B2 antibody [EPR11250] (ab157479) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Human testis lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 26 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
      Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
      Immunofluorescent analysis of K562 cells labeling PAFAH1B2 with ab157479 at 1/50 dilution.
    • Flow Cytometry - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
      Flow Cytometry - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
      Flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B2 with ab157479 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab157479 has not yet been referenced specifically in any publications.

    Publishing research using ab157479? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab157479.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up