Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-PAFAH1B2 antibody [EPR11250]
See all PAFAH1B2 primary antibodies -
DescriptionRabbit monoclonal [EPR11250] to PAFAH1B2
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Recombinant fragment corresponding to residues in Human PAFAH1B2 (UniProt P68402).
-
Positive control
- SH-SY5Y, 293T, Human testis and K562 lysates; K562 cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20ºC.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR11250
-
IsotypeIgG
-
Research areas
Associated products
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 26 kDa. | |
| ICC/IF | 1/50 - 1/100. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
-
FunctionInactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
-
Tissue specificityUbiquitous.
-
Sequence similaritiesBelongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
-
Cellular localizationCytoplasm.
- Information by UniProt
-
Database links
- Entrez Gene: 5049 Human
- Entrez Gene: 18475 Mouse
- Entrez Gene: 64189 Rat
- Omim: 602508 Human
- SwissProt: P68402 Human
- SwissProt: Q61206 Mouse
- SwissProt: O35264 Rat
- Unigene: 597488 Human
see all -
Alternative names
- Intracellular platelet activating factor acetylhydrolase alpha 2 subunit antibody
- PA1B2_HUMAN antibody
- PAF acetylhydrolase 30 kDa subunit antibody
see all
Images
-
![Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)](https://www.abcam.com/ps/datasheet/images/157/ab157479/PAFAH1B2-Primary-antibodies-ab157479-1.jpg)
Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)All lanes : Anti-PAFAH1B2 antibody [EPR11250] (ab157479) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Human testis lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)](https://www.abcam.com/ps/datasheet/images/157/ab157479/PAFAH1B2-Primary-antibodies-ab157479-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)Immunofluorescent analysis of K562 cells labeling PAFAH1B2 with ab157479 at 1/50 dilution. -
![Flow Cytometry - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)](https://www.abcam.com/ps/datasheet/images/157/ab157479/PAFAH1B2-Primary-antibodies-ab157479-3.jpg)
Flow Cytometry - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)Flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B2 with ab157479 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Datasheets and documents
Protocols
References
ab157479 has not yet been referenced specifically in any publications.
