Recombinant Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
![Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)](https://www.abcam.com/ps/products/157/ab157479/Images/ab157479-164554-anti-pafah1b2-antibody-epr11250-western-blot.jpg "Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11250] to PAFAH1B2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PAFAH1B2 antibody [EPR11250]
See all PAFAH1B2 primary antibodies -
Description
Rabbit monoclonal [EPR11250] to PAFAH1B2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human PAFAH1B2.
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Positive control
- SH-SY5Y, 293T, Human testis and K562 lysates; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11250 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
|
|
| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 26 kDa. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5049 Human
- Entrez Gene: 18475 Mouse
- Entrez Gene: 64189 Rat
- Omim: 602508 Human
- SwissProt: P68402 Human
- SwissProt: Q61206 Mouse
- SwissProt: O35264 Rat
- Unigene: 597488 Human
see all -
Alternative names
- Intracellular platelet activating factor acetylhydrolase alpha 2 subunit antibody
- PA1B2_HUMAN antibody
- PAF acetylhydrolase 30 kDa subunit antibody
see all
Images
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Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)All lanes : Anti-PAFAH1B2 antibody [EPR11250] (ab157479) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Human testis lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)](https://www.abcam.com/ps/products/157/ab157479/Images/ab157479-164553-anti-pafah1b2-antibody-epr11250-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)Immunofluorescent analysis of K562 cells labeling PAFAH1B2 with ab157479 at 1/50 dilution. -
![Flow Cytometry (Intracellular) - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)](https://www.abcam.com/ps/products/157/ab157479/Images/ab157479-164552-anti-pafah1b2-antibody-epr11250-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)Intracellular flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B2 with ab157479 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
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![Anti-PAFAH1B2 antibody [EPR11250] (ab157479)](https://www.abcam.com/ps/products/157/ab157479/Images/ab157479-4-benefits-of-recombinant-antibodies.png)
Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab157479 has been referenced in 1 publication.
- Lin Q et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468
