Product nameAnti-PAFAH1B2 antibody [EPR11250]
See all PAFAH1B2 primary antibodies
DescriptionRabbit monoclonal [EPR11250] to PAFAH1B2
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to residues in Human PAFAH1B2 (UniProt P68402).
- SH-SY5Y, 293T, Human testis and K562 lysates; K562 cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab157479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 26 kDa.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
Sequence similaritiesBelongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
- Information by UniProt
- Intracellular platelet activating factor acetylhydrolase alpha 2 subunit antibody
- PA1B2_HUMAN antibody
- PAF acetylhydrolase 30 kDa subunit antibody
All lanes : Anti-PAFAH1B2 antibody [EPR11250] (ab157479) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Human testis lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Immunofluorescent analysis of K562 cells labeling PAFAH1B2 with ab157479 at 1/50 dilution.
Flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B2 with ab157479 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
ab157479 has not yet been referenced specifically in any publications.