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  1. Link

    pafah1b2-antibody-epr11250-ab157479.pdf

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RecombinantRabMAb

Recombinant Anti-PAFAH1B2 antibody [EPR11250] (ab157479)

  • Datasheet
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Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
  • Flow Cytometry (Intracellular) - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
  • Anti-PAFAH1B2 antibody [EPR11250] (ab157479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11250] to PAFAH1B2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-PAFAH1B2 antibody [EPR11250]
    See all PAFAH1B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11250] to PAFAH1B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human PAFAH1B2.

  • Positive control

    • SH-SY5Y, 293T, Human testis and K562 lysates; K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11250
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-PAFAH1B2 antibody [EPR11250] - BSA and Azide free (ab249342)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
ICC/IF
1/50 - 1/100.
Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P68402 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5049 Human
    • Entrez Gene: 18475 Mouse
    • Entrez Gene: 64189 Rat
    • Omim: 602508 Human
    • SwissProt: P68402 Human
    • SwissProt: Q61206 Mouse
    • SwissProt: O35264 Rat
    • Unigene: 597488 Human
    • Unigene: 200859 Mouse
    • Unigene: 24751 Rat
    see all
  • Alternative names

    • Intracellular platelet activating factor acetylhydrolase alpha 2 subunit antibody
    • PA1B2_HUMAN antibody
    • PAF acetylhydrolase 30 kDa subunit antibody
    • PAF AH 30 kDa subunit antibody
    • PAF AH beta subunit antibody
    • PAF AH subunit beta antibody
    • PAF-AH 30 kDa subunit antibody
    • PAF-AH subunit beta antibody
    • PAFAH 30 kDa subunit antibody
    • PAFAH beta subunit antibody
    • PAFAH subunit beta antibody
    • Pafah1b2 antibody
    • PAFAH1B2 protein antibody
    • PAFAHB antibody
    • Platelet activating factor acetylhydrolase 1b catalytic subunit 2 antibody
    • Platelet activating factor acetylhydrolase IB beta subunit antibody
    • Platelet activating factor acetylhydrolase IB subunit beta antibody
    • Platelet activating factor acetylhydrolase isoform Ib beta subunit antibody
    • Platelet activating factor acetylhydrolase isoform Ib subunit 2 antibody
    • Platelet-activating factor acetylhydrolase IB subunit beta antibody
    see all

Images

  • Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
    Western blot - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
    All lanes : Anti-PAFAH1B2 antibody [EPR11250] (ab157479) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Human testis lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
    Immunocytochemistry/ Immunofluorescence - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
    Immunofluorescent analysis of K562 cells labeling PAFAH1B2 with ab157479 at 1/50 dilution.
  • Flow Cytometry (Intracellular) - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
    Flow Cytometry (Intracellular) - Anti-PAFAH1B2 antibody [EPR11250] (ab157479)

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B2 with ab157479 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

     

  • Anti-PAFAH1B2 antibody [EPR11250] (ab157479)
    Anti-PAFAH1B2 antibody [EPR11250] (ab157479)

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab157479? Please let us know so that we can cite the reference in this datasheet.

ab157479 has been referenced in 1 publication.

  • Lin Q  et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468

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