Anti-PAFAH1B3 antibody (ab241288)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PAFAH1B3 antibody
See all PAFAH1B3 primary antibodies -
Description
Rabbit polyclonal to PAFAH1B3 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PAFAH1B3 aa 181-231. The exact sequence is proprietary.
Database link: Q15102 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to PAFAH1B3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. |
Target
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Function
Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain. -
Tissue specificity
In the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain. -
Sequence similarities
Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5050 Human
- Omim: 603074 Human
- SwissProt: Q15102 Human
- Unigene: 466831 Human
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Alternative names
- PA1B3_HUMAN antibody
- PAF acetylhydrolase 29 kDa subunit antibody
- PAF AH 29 kDa subunit antibody
see all
Images
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Immunoprecipitation - Anti-PAFAH1B3 antibody (ab241288)PAFAH1B3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded prepared using NETN lysis buffer) using ab241288 at 6 µg per reaction. Western bot was performed on the immunoprecipitate using ab241288 at 0.4 µg/ml.
Lane 1: ab241288 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-PAFAH1B3 antibody (ab241288)All lanes : Anti-PAFAH1B3 antibody (ab241288) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
Datasheets and documents
References
ab241288 has not yet been referenced specifically in any publications.
