Key features and details
- Rabbit polyclonal to PAFAH1B3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PAFAH1B3 antibody
See all PAFAH1B3 primary antibodies
DescriptionRabbit polyclonal to PAFAH1B3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human PAFAH1B3 aa 1-50. The exact sequence is proprietary.
Database link: Q15102
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241362 was affinity purified using an epitope specific to PAFAH1B3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 26 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.
Tissue specificityIn the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain.
Sequence similaritiesBelongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
- Information by UniProt
- PA1B3_HUMAN antibody
- PAF acetylhydrolase 29 kDa subunit antibody
- PAF AH 29 kDa subunit antibody
All lanes : Anti-PAFAH1B3 antibody (ab241362) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 10 seconds
PAFAH1B3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241362 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241362 at 0.4 µg/ml.
Lane 1: ab241362 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Lysate prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241362 has not yet been referenced specifically in any publications.