Overview

  • Product name

  • Description

    Rabbit polyclonal to PAFAH1B3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human PAFAH1B3 aa 1-50. The exact sequence is proprietary.
    Database link: Q15102

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 26 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.
  • Tissue specificity

    In the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain.
  • Sequence similarities

    Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • PA1B3_HUMAN antibody
    • PAF acetylhydrolase 29 kDa subunit antibody
    • PAF AH 29 kDa subunit antibody
    • PAF AH subunit gamma antibody
    • PAF-AH 29 kDa subunit antibody
    • PAF-AH subunit gamma antibody
    • PAFAH subunit gamma antibody
    • Pafah1b3 antibody
    • PAFAHG antibody
    • Platelet activating factor acetylhydrolase IB subunit gamma antibody
    • Platelet activating factor acetylhydrolase isoform Ib gamma subunit (29kD) antibody
    • Platelet activating factor acetylhydrolase isoform Ib gamma subunit 29kDa antibody
    • Platelet activating factor acetylhydrolase isoform Ib gamma subunit antibody
    • Platelet-activating factor acetylhydrolase IB subunit gamma antibody
    see all

Images

  • All lanes : Anti-PAFAH1B3 antibody (ab241362) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 26 kDa


    Exposure time: 10 seconds
  • PAFAH1B3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241362 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241362 at 0.4 µg/ml.

    Lane 1: ab241362 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

    Lysate prepared using NETN lysis buffer.

References

ab241362 has not yet been referenced specifically in any publications.

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