Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-PAFAH1B3 antibody [EPR11231-10-6]
See all PAFAH1B3 primary antibodies -
DescriptionRabbit monoclonal [EPR11231-10-6] to PAFAH1B3
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PAFAH1B3. The exact sequence is proprietary.
Database link: Q15102 -
Positive control
- HeLa, 293T, U87MG and K562 cell lysates, K562 cells
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR11231-10-6
-
IsotypeIgG
-
Research areas
Associated products
-
Isotype control
-
Positive Controls
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab170877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 26 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
-
FunctionInactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.
-
Tissue specificityIn the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain.
-
Sequence similaritiesBelongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
-
Cellular localizationCytoplasm.
- Information by UniProt
-
Database links
- Entrez Gene: 5050 Human
- Omim: 603074 Human
- SwissProt: Q15102 Human
- Unigene: 466831 Human
-
Alternative names
- PA1B3_HUMAN antibody
- PAF acetylhydrolase 29 kDa subunit antibody
- PAF AH 29 kDa subunit antibody
see all
Images
-
![Flow Cytometry - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)](https://www.abcam.com/ps/products/170/ab170877/Images/ab170877-171342-ab170877-2.jpg)
Flow Cytometry - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)Flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B3using ab170877 at a 1/10 dilution (red) or a rabbit IgG (green).
-
![Western blot - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)](https://www.abcam.com/ps/products/170/ab170877/Images/ab170877-171341-ab170877.jpg)
Western blot - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)All lanes : Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87MG cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 26 kDa
Datasheets and documents
References
ab170877 has not yet been referenced specifically in any publications.
