Recombinant Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)
![Flow Cytometry (Intracellular) - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)](https://www.abcam.com/ps/products/170/ab170877/Images/ab170877-171342-ab170877-2.jpg "Flow Cytometry (Intracellular) - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11231-10-6] to PAFAH1B3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PAFAH1B3 antibody [EPR11231-10-6]
See all PAFAH1B3 primary antibodies -
Description
Rabbit monoclonal [EPR11231-10-6] to PAFAH1B3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PAFAH1B3. The exact sequence is proprietary.
Database link: Q15102 -
Positive control
- HeLa, 293T, U87MG and K562 cell lysates, K562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11231-10-6 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
Target
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Function
Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain. -
Tissue specificity
In the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain. -
Sequence similarities
Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5050 Human
- Omim: 603074 Human
- SwissProt: Q15102 Human
- Unigene: 466831 Human
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Alternative names
- PA1B3_HUMAN antibody
- PAF acetylhydrolase 29 kDa subunit antibody
- PAF AH 29 kDa subunit antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)
Intracellular flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B3using ab170877 at a 1/10 dilution (red) or a rabbit IgG (green).
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![Western blot - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)](https://www.abcam.com/ps/products/170/ab170877/Images/ab170877-171341-ab170877.jpg)
Western blot - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)All lanes : Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87MG cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 26 kDa -
![Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)](https://www.abcam.com/ps/products/170/ab170877/Images/ab170877-3-benefits-of-recombinant-antibodies.png)
Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)
Datasheets and documents
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Datasheet download
References (1)
ab170877 has been referenced in 1 publication.
- Fan J et al. Aberrant Expression of PAFAH1B3 Affects Proliferation and Apoptosis in Osteosarcoma. Front Oncol 11:664478 (2021). PubMed: 34136395
