Recombinant Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11231-10-6] to PAFAH1B3
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PAFAH1B3 antibody [EPR11231-10-6]
See all PAFAH1B3 primary antibodies -
Description
Rabbit monoclonal [EPR11231-10-6] to PAFAH1B3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PAFAH1B3. The exact sequence is proprietary.
Database link: Q15102 -
Positive control
- HeLa, 293T, U87MG and K562 cell lysates, K562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11231-10-6 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 26 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain. -
Tissue specificity
In the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain. -
Sequence similarities
Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5050 Human
- Omim: 603074 Human
- SwissProt: Q15102 Human
- Unigene: 466831 Human
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Alternative names
- PA1B3_HUMAN antibody
- PAF acetylhydrolase 29 kDa subunit antibody
- PAF AH 29 kDa subunit antibody
see all
Images
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![Flow Cytometry - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)](https://www.abcam.com/ps/products/170/ab170877/Images/ab170877-171342-ab170877-2.jpg)
Flow Cytometry - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)Flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B3using ab170877 at a 1/10 dilution (red) or a rabbit IgG (green).
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![Western blot - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)](https://www.abcam.com/ps/products/170/ab170877/Images/ab170877-171341-ab170877.jpg)
Western blot - Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)All lanes : Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87MG cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 26 kDa -
![Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)](https://www.abcam.com/ps/products/170/ab170877/Images/ab170877-3-benefits-of-recombinant-antibodies.png)
Anti-PAFAH1B3 antibody [EPR11231-10-6] (ab170877)
Datasheets and documents
References (0)
ab170877 has not yet been referenced specifically in any publications.
