Product nameAnti-PAG antibody [EPR9705]
See all PAG primary antibodies
DescriptionRabbit monoclonal [EPR9705] to PAG
Tested applicationsSuitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PAG. The exact sequence is proprietary.
- Raji, Jurkat, HeLa and Human placenta lysate; Human tonsil tissue
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab155100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
Csk binding protein is an ubiquitously expressed 47 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. PAG has a pI value of about 4.6 and binds poorly to SDS and therefore has decreased mobility on SDS gels.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionNegatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
Tissue specificityUbiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
Cellular localizationCell membrane. Present in lipid rafts.
- Information by UniProt
- CBP antibody
- Csk binding protein antibody
- Csk-binding protein antibody
All lanes : Anti-PAG antibody [EPR9705] (ab155100) at 1/1000 dilution
Lane 1 : Raji lysate
Lane 2 : Jurkat lysate
Lane 3 : HeLa lysate
Lane 4 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 47 kDa
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling PAG with ab155100 at a 1/100 dilution.
This product has been referenced in:
- Shen L et al. PAG1 promotes the inherent radioresistance of laryngeal cancer cells via activation of STAT3. Exp Cell Res 370:127-136 (2018). Read more (PubMed: 29913153) »
- Moogk D et al. Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets. J Immunol 197:644-54 (2016). Read more (PubMed: 27271569) »