Overview

  • Product name
    Anti-PAG antibody [EPR9705]
    See all PAG primary antibodies
  • Description
    Rabbit monoclonal [EPR9705] to PAG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PAG. The exact sequence is proprietary.

  • Positive control
    • Raji, Jurkat, HeLa and Human placenta lysate; Human tonsil tissue
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.

Csk binding protein is an ubiquitously expressed 47 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. PAG has a pI value of about 4.6 and binds poorly to SDS and therefore has decreased mobility on SDS gels.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
    • Tissue specificity
      Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
    • Post-translational
      modifications
      Palmitoylated.
      Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
    • Cellular localization
      Cell membrane. Present in lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • CBP antibody
      • Csk binding protein antibody
      • Csk-binding protein antibody
      • FLJ37858 antibody
      • MGC138364 antibody
      • PAG 1 antibody
      • PAG antibody
      • PAG1 antibody
      • PAG1_HUMAN antibody
      • Phosphoprotein associated with glycosphingolipid enriched microdomains 1 antibody
      • Phosphoprotein associated with glycosphingolipid enriched microdomains antibody
      • Phosphoprotein associated with glycosphingolipid microdomains 1 antibody
      • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 antibody
      • Protein Associated with Glycosphingolipid Enriched Microdomains antibody
      • Transmembrane adapter protein PAG antibody
      • Transmembrane phosphoprotein Cbp antibody
      see all

    Images

    • All lanes : Anti-PAG antibody [EPR9705] (ab155100) at 1/1000 dilution

      Lane 1 : Raji lysate
      Lane 2 : Jurkat lysate
      Lane 3 : HeLa lysate
      Lane 4 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 47 kDa

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling PAG with ab155100 at a 1/100 dilution.

    References

    This product has been referenced in:
    • Moogk D  et al. Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets. J Immunol 197:644-54 (2016). Read more (PubMed: 27271569) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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