Overview

  • Product name
    Anti-PAI1 antibody [1D5]
    See all PAI1 primary antibodies
  • Description
    Mouse monoclonal [1D5] to PAI1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Purified recombinant fragment of Human PAI1 expressed in E. Coli.

  • Positive control
    • Recombinant Human PAI1 protein (aa194-316); PAI1 (aa194-316)-hIgGFc transfected HEK293 cell lysate; Human lung cancer and kidney cancer tissues; NIH 3T3 cells.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR201079-27 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab125687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 45 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/200 - 1/1000.

Target

  • Function
    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    modifications
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Images

  • Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution + Human PAI1 recombinant protein (amino acids 194-316)

    Predicted band size: 45 kDa

  • All lanes : Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PAI1-hIgGFc transfected HEK293 cell lysate (aa194-316)

    Predicted band size: 45 kDa

  • ELISA using ab125687.
  • ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human lung cancer tissue by Immunohistochemistry followed by DAB staining.
  • ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human kidney cancer tissue by Immunohistochemistry followed by DAB staining.
  • ab125687 at 1/200 dilution staining PAI1 in NIH 3T3 cells by Flow Cytometry (green), negative control (red).

References

This product has been referenced in:
  • Li X  et al. Encapsulated papillary carcinoma of the breast: A clinicopathological study of 49 cases. Curr Probl Cancer N/A:N/A (2018). Read more (PubMed: 29731165) »
  • Peterle GT  et al. PAI-1, CAIX, and VEGFA expressions as prognosis markers in oral squamous cell carcinoma. J Oral Pathol Med 47:566-574 (2018). Read more (PubMed: 29693741) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

The price for antibody ab125687 is €390 plus €39 shipping charges to Switzerland.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your reply.

As ab125687 PAI1 antibody [1D5], ab1834 Factor XIIIa antibody [AC-1A1] and ab54488 CD41/Integrin alpha 2b antibody [283.16B7] are all mouse IgG1, Ican suggest to consider the following anti mouse IgG1 secondaries available in our catalog. These are biotin conjugated and have been tested and covered by our guarantee for IHC-Fr. I can recommend to review the online datasheets for further information and ensure they are suitable for your purposes:

ab98691 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (Biotin), pre-adsorbed
Biotin conjugated
Tested in: ELISA, ICC/IF, IHC-P, WB
Reacts with: Mouse
https://www.abcam.com/index.html?datasheet=98691 (or use the following: https://www.abcam.com/index.html?datasheet=98691).

ab97238 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (Biotin)
Biotin conjugated ELISA, ICC/IF, IHC-P, WB
Reacts with: Mouse
https://www.abcam.com/index.html?datasheet=97238 (or use the following: https://www.abcam.com/index.html?datasheet=97238).


Once you have decided which of these you would like to order, please email my colleague Andrew again (mailto:andrew.richards@abcam.com) with your choice and he will be pleased to place the order for you.

Please note I am out of the office next week, so I can suggest to contact Andrew if you have any further questions regarding the discounts or the order.

The scientific support team are happy to help if you have any further technical questions (mailto:technical@abcam.com).

I hope this provides a satisfactory outcome for you and I wish you a good bank holiday weekend. Good luck with your research.

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Answer

Merci de votre question et votre intérêt pour nos produits.

L'anticorps secondaire ab98689 n'est pas couplé à la biotine - c'est juste l'anticorps secondaire non-conjugué. Les anticorps pré-adsorbés sont des anticorps pour lesquels la réactivité contre des immunoglobulines des espèces homologues avait été enlevé. Ces anticorps sont prêt à l'emploi - ils doivent toute fois toujours être dilués. Le grand avantage est pour ces anticorps qu'ils vont avoir une crossreactivité minimale avec des autres Ig et sont donc très utiles pour diminuer le bruit de fond ou pour des doubles ou multiplesmarquages. Le ab98689 est préabsorbé contre les protéines humaines et de rat comme indiqué sur la fiche technique sous "minimal crossreactivity".

Comme anticorps secondaire biotinylé contre les Ig ou les IgG1 de souris, je peux vous recommander les anticorps suivants:

-le ab97033goat to mouse IgG H&L, preabsorbed, Biotin conjugated - Cet anticorps va reconnaitre la chaine lourde et la chaine légère (H&L) des immunoglobulines de souris, et il avait été préabsorbé contre les protéines de poulot, bovine, chèvre, cheval, humain, lapin, rat et mouton https://www.abcam.com/index.html?datasheet=97033 (or use the following: https://www.abcam.com/index.html?datasheet=97033).

-ab97021, goat to mouse IgG H&L, preabsorbed, Biotin conjugated -Cet anticorps n'avais pas été préabsorbé. https://www.abcam.com/index.html?datasheet=97021 (or use the following: https://www.abcam.com/index.html?datasheet=97021).

Si vous préférez un anticorps contre l'IgG1, je peux vous recommander le ab98691, un goat anti mouse IgG1, Biotin, préabsorbed https://www.abcam.com/index.html?datasheet=98691 (or use the following: https://www.abcam.com/index.html?datasheet=98691).

Pour l'anticorps secondaire contre les Ig de lapin, je peux vous recommanderl'anticorpssuivant:

-ab98497 donkeytorabbit IgG H&L, preabsorbed, Biotin conjugated - Cet anticorps va reconnaitre la chaine lourde et la chaine légère (H&L) des immunoglobulines de lapin, et il avait été préabsorbé contre les protéines humaines, de souris et de rat https://www.abcam.com/index.html?datasheet=98497 (or use the following: https://www.abcam.com/index.html?datasheet=98497).

Ma collègue va vous recontacter avec un nouveau devis - incluant le ab97033 et le ab98497. Veuillez ne pas hésiter de nous recontacter si vous préférez des autres anticorps secondaires et si vous avez des autres produits à ajouter sur le devis.

J'espère que ces informations vous sont utiles. N'hésitez pas à nous recontacter si vous avez besoin d'autres renseignements ou conseils.

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