The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.5 - 3 µg/ml.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Use a concentration of 4 - 6 µg/ml.
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen. Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
Belongs to the serpin family.
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg- -Met-370 bond.
Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
Serpin E1 antibody
Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
Serpin peptidase inhibitor clade E antibody
Serpine 1 antibody
Western blot - Anti-PAI1 antibody (ab31280)
Anti-PAI1 antibody (ab31280) at 0.1 µg/ml + Human Liver lysate at 35 µg
Predicted band size: 45 kDa Observed band size: 45 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab31280)This image is courtesy of an anonymous Abreview
ab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody.
Tsuge M et al. SK-216, a Novel Inhibitor of Plasminogen Activator Inhibitor-1, Suppresses Lung Metastasis of Human Osteosarcoma. Int J Mol Sci19:N/A (2018).
Read more (PubMed: 29510576) »
Hirahata M et al. PAI-1, a target gene of miR-143, regulates invasion and metastasis by upregulating MMP-13 expression of human osteosarcoma. Cancer Med5:892-902 (2016).
Read more (PubMed: 26817521) »