Anti-PAI1 antibody (ab7205)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameAnti-PAI1 antibody
See all PAI1 primary antibodies -
DescriptionRabbit polyclonal to PAI1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, ICC, WBmore details
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Species reactivityReacts with: Mouse, Rat
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Immunogen
Recombinant full length protein (Rat).
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Positive control
- This antibody gave a positive signal in ICC/IF in MEF1 cell line.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab7205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| ICC | 1/200. | |
| WB | 1/1000. |
Target
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FunctionThis inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
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Tissue specificityFound in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
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Involvement in diseaseDefects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
Sequence similaritiesBelongs to the serpin family.
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Post-translational
modificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 18787 Mouse
- Entrez Gene: 24617 Rat
- SwissProt: P22777 Mouse
- SwissProt: P20961 Rat
- Unigene: 250422 Mouse
- Unigene: 29367 Rat
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Alternative names
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
Images
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Western blot - Anti-PAI1 antibody (ab7205)Anti-PAI1 antibody (ab7205) at 1/1000 dilution
Rat PAI1 detected by Western blot on Day 3 in a glomerular nephritis model. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab7205)ICC/IF image of ab7205 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7205, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Li B et al. MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1. Exp Ther Med 15:5125-5132 (2018). Read more (PubMed: 29805539) »
- Stefos GC et al. NGF upregulates the plasminogen activation inhibitor-1 in neurons via the Calcineurin/NFAT pathway and the down syndrome-related proteins DYRK1A and RCAN1 attenuate this effect. PLoS One 8:e67470 (2013). WB . Read more (PubMed: 23825664) »
