RecombinantRabMAb

Recombinant Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)

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Immunoprecipitation - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
  • Flow Cytometry - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
  • Western blot - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
  • Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21850-82] to PAI1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free
    See all PAI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21850-82] to PAI1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human PAI1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P05121

  • Positive control

    • ICC/IF: HUVEC cells. WB: Mouse and rat placenta lysate. Rat lung lysate.

  • General notes

    Ab237780 is the carrier-free version of ab222754. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab237780 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab237780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

  • Sequence similarities

    Belongs to the serpin family.

  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • Nexin plasminogen activator inhibitor type 1 antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serine proteinase inhibitor clade E member 1 antibody
    • serpin antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Images

  • Immunoprecipitation - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
    Immunoprecipitation - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)

    PAI1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell line) whole cell lysate with ab222754 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222754 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution.
    Lane 1: HepG2 whole cell lysate 10 µg (Input).
    Lane 2: ab222754 IP in HepG2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222754 in HepG2 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222754).

  • Flow Cytometry - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
    Flow Cytometry - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 cells serum-starved for 4 hours, treated with TGF-β (10 ng/ml) for 3 hours, and then with TGF-β (10 ng/ml) and BFA (300 ng/ml) together for 18 hours (Red) / Untreated control (Green) labeling PAI1 with ab222754 at 1/60 (red/green) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    The expression of PAI-1 is induced by TGF-β in NIH/3T3 cell line (PMID 17890327).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222754).

  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
    Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling PAI1 with ab222754 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing the signal is increased in 4 hour serum-starved NIH/3T3 cells treated with TGF-β (10 ng/ml) for 3 hours, then with TGF-β (10 ng/ml) and BFA (300 ng/ml) together for 18 hours. The expression of PAI-1 is induced by TGF-β in the NIH/3T3 cell line (PMID 17890327). The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222754).

  • Western blot - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
    Western blot - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
    All lanes : Anti-HIF-1 alpha antibody [EPR16897] (ab179483) at 1/1000 dilution

    Lane 1 : Mouse placenta tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat placenta tissue lysate
    Lane 5 : Rat lung tissue lysate
    Lane 6 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa
    Additional bands at: 37 kDa (possible non-specific binding)


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression levels of mouse and rat PAI1 may be low in normal liver tissue (PMID: 21898503). This antibody detects a 37 kDa extra band and no specific band in mouse liver and no bands in rat liver.
    Although lung tissue is reported to be PAI1 positive (PMID: 21768189, PMID: 17032919), this antibody can’t detect band of target in mouse lung and detects weak target band in rat lung.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179483).

  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
    Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (Human umbilical vein endothelial cell line) cells labeling PAI1 with ab222754 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing cytoplasmic staining in HUVEC cell line. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab222754).

  • Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)
    Anti-PAI1 antibody [EPR21850-82] - BSA and Azide free (ab237780)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab237780? Please let us know so that we can cite the reference in this datasheet.

ab237780 has not yet been referenced specifically in any publications.

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