Overview

  • Product name

    Anti-PAI1 antibody [I-3]
    See all PAI1 primary antibodies
  • Description

    Mouse monoclonal [I-3] to PAI1
  • Host species

    Mouse
  • Specificity

    ab82218 is specific for human PAI-1. No cross-reactivity is observed with PAI-2 as tested by ELISA and immunoblotting. No reactivity is observed with any other human plasma protein when tested by immunoblotting.
  • Tested applications

    Suitable for: WB, IHC-Fr, Indirect ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Partialy purified human PAI-1

  • Epitope

    ab82218 binds to an epitope consisting of amino acids 235-283

Properties

Applications

Our Abpromise guarantee covers the use of ab82218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-Fr Use at an assay dependent concentration.
Indirect ELISA Use at an assay dependent concentration.

Target

  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • Nexin plasminogen activator inhibitor type 1 antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serine proteinase inhibitor clade E member 1 antibody
    • serpin antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

References

This product has been referenced in:

  • Gopal S  et al. Estrogen regulates endothelial migration via plasminogen activator inhibitor (PAI-1). Mol Hum Reprod 18:410-6 (2012). WB ; Rat . Read more (PubMed: 22389473) »
  • Marchand A  et al. miR-421 and miR-30c inhibit SERPINE 1 gene expression in human endothelial cells. PLoS One 7:e44532 (2012). WB . Read more (PubMed: 22952991) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (T47D breast cancer cell)
Loading amount
40 µg
Specification
T47D breast cancer cell
Treatment
17 beta estradiol 10 nM Time course experiment
Gel Running Conditions
Reduced Denaturing (8)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. santhosh gopal

Verified customer

Submitted Jul 26 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up