Product namePAI1 overexpression 293T lysate (whole cell)
See all PAI1 lysates
DescriptionPAI1 overexpression lysate
ab94071 is a 293T cell transfected lysate in which Human PAI1 has been transiently over-expressed using a pCMV-PAI1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundDisease: Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen. Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. Function: This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. PTM: Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg- -Met-370 bond. Similarity: Belongs to the serpin family. Tissue specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Our Abpromise guarantee covers the use of ab94071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94071 at 15µg/lane on an SDS-PAGE gel
All lanes : Anti-PAI1 antibody (ab89581) at 1/500 dilution
Lane 1 :
PAI1 overexpression 293T lysate (whole cell) (ab94071)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94071 has not yet been referenced specifically in any publications.