PAI1 Activity Assay Kit (Colorimetric) (ab283368)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Inhibitor compounds
- Sensitivity: 2 U/ml
Overview
-
Product name
PAI1 Activity Assay Kit (Colorimetric)
See all PAI1 kits -
Detection method
Colorimetric -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity (quantitative) -
Sensitivity
2 U/ml -
Assay duration
Multiple steps standard assay -
Product overview
The Plasminogen Activator Inhibitor-1 Activity Assay Kit is a two-step colorimetric assay. In the first step, samples are incubated with a known amount of tPA thereby allowing PAI-1 and tPA to form an inactive complex. In the second step, the residual free tPA converts plasminogen to plasmin, which in turn hydrolyzes the plasmin chromogenic substrate thereby releasing pNA (chromophore). The absorbance of the released pNA is inversely proportional to the PAI-1 activity in the samples. The assay kit is simple, specific and can detect as low as 2 U/ml of PAI-1 activity in samples.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K2040 Plasminogen Activator Inhibitor-1 Activity Assay Kit (C). K2040-100 is the same size as the 100 test size of ab283368.
Plasminogen Activator Inhibitor-1 (PAI-1), also known as endothelial plasminogen activator inhibitor, is a serine protease inhibitor that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA). PAI-1 is present in a number of tissues & cell types and is associated with various thrombotic and fibrinolytic complications. High level of PAI-1 activity is found in patients suffering from myocardial infarction, hemolytic uremic syndrome or stroke whereas low levels of PAI-1 activity are associated with bleeding disorders. Additionally, PAI-1 is a prognosticator in various cancers.
-
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 96-Well Half Area Plate 1 unit Active Human tPA 1 vial PAI-1 Acidification Buffer 1 x 5ml PAI-1 Assay Buffer 1 x 25ml PAI-1 Stop Buffer 1 x 5ml PAI-1 Substrate Mix 1 vial Plasminogen 1 vial -
Research areas
-
Function
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab283368 has not yet been referenced specifically in any publications.