Anti-PAICS/PAIS antibody (ab224155)
Key features and details
- Rabbit polyclonal to PAICS/PAIS
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-PAICS/PAIS antibody
See all PAICS/PAIS primary antibodies -
Description
Rabbit polyclonal to PAICS/PAIS -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human PAICS/PAIS aa 50-200.
Database link: P22234 -
Positive control
- WB: RT4, U-251 MG, NIH/3T3 and NBT-II cell lysates. IHC-P: Human colon, tonsil and duodenum tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47,48 kDa.
|
Notes |
---|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47,48 kDa. |
Target
-
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1. -
Sequence similarities
In the N-terminal section; belongs to the SAICAR synthetase family.
In the C-terminal section; belongs to the AIR carboxylase family. - Information by UniProt
-
Database links
- Entrez Gene: 10606 Human
- Entrez Gene: 67054 Mouse
- Entrez Gene: 140946 Rat
- Omim: 172439 Human
- SwissProt: P22234 Human
- SwissProt: Q9DCL9 Mouse
- SwissProt: P51583 Rat
- Unigene: 518774 Human
see all -
Alternative names
- ADE2 antibody
- ADE2H1 antibody
- AIR carboxylase antibody
see all
Images
-
Paraffin-embedded human duodenum tissue stained for PAICS/PAIS using ab224155 at 1/50 dilution in immunohistochemical analysis.
-
Paraffin-embedded human tonsil tissue stained for PAICS/PAIS using ab224155 at 1/50 dilution in immunohistochemical analysis.
-
Paraffin-embedded human liver tissue stained for PAICS/PAIS using ab224155 at 1/50 dilution in immunohistochemical analysis.
-
All lanes : Anti-PAICS/PAIS antibody (ab224155) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 47,48 kDa -
All lanes : Anti-PAICS/PAIS antibody (ab224155) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 47,48 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab224155 has not yet been referenced specifically in any publications.