Key features and details
- Rabbit polyclonal to PAICS/PAIS
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-PAICS/PAIS antibody
See all PAICS/PAIS primary antibodies
DescriptionRabbit polyclonal to PAICS/PAIS
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
Recombinant fragment within Human PAICS/PAIS (internal sequence). The exact sequence is proprietary.
Database link: P22234
- WB: Jurkat whole cell lysate. IHC-P: RT2 xenograft and HBL435 xenograft tissues.
This product was previously labelled as PAICS
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 47 kDa.|
PathwayPurine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Sequence similaritiesIn the N-terminal section; belongs to the SAICAR synthetase family.
In the C-terminal section; belongs to the AIR carboxylase family.
- Information by UniProt
- ADE2 antibody
- ADE2H1 antibody
- AIR carboxylase antibody
Anti-PAICS/PAIS antibody (ab229531) at 1/10000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS-PAGE gel.
Paraffin-embedded RT2 xenograft tissue stained for PAICS/PAIS using ab229531 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded HBL435 xenograft stained for PAICS/PAIS using ab229531 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229531 has not yet been referenced specifically in any publications.