Product nameAnti-PAICS/PAIS antibody
See all PAICS/PAIS primary antibodies
DescriptionRabbit polyclonal to PAICS/PAIS
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human PAICS/PAIS aa 1-50. The exact sequence is proprietary. (NP_001072992.1).
Database link: P22234
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: PAICS/PAIS in HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
This product was previously labelled as PAICS
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
PathwayPurine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Sequence similaritiesIn the N-terminal section; belongs to the SAICAR synthetase family.
In the C-terminal section; belongs to the AIR carboxylase family.
- Information by UniProt
- ADE2 antibody
- ADE2H1 antibody
- AIR carboxylase antibody
All lanes : Anti-PAICS/PAIS antibody (ab240743) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for PAICS/PAIS using ab240743 at 1 µg/ml in immunohistochemical analysis. DAB staining.
PAICS/PAIS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240743 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240743 at 0.4 µg/ml.
Lane 1: ab240743 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab240743 has not yet been referenced specifically in any publications.