Overview

  • Product name

  • Description

    Rabbit polyclonal to PAICS/PAIS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human PAICS/PAIS aa 1-50. The exact sequence is proprietary. (NP_001072992.1).
    Database link: P22234

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: PAICS/PAIS in HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
  • General notes

     This product was previously labelled as PAICS

     

Properties

Applications

Our Abpromise guarantee covers the use of ab240743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway

    Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
    Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
  • Sequence similarities

    In the N-terminal section; belongs to the SAICAR synthetase family.
    In the C-terminal section; belongs to the AIR carboxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADE2 antibody
    • ADE2H1 antibody
    • AIR carboxylase antibody
    • AIRC antibody
    • DKFZp781N1372 antibody
    • MGC1343 antibody
    • MGC5024 antibody
    • Multifunctional protein ADE2 antibody
    • Multifunctional protein ADE2H1 antibody
    • Paics antibody
    • PAIS antibody
    • Phosphoribosylaminoimidazole carboxylase antibody
    • Phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase antibody
    • PUR6_HUMAN antibody
    • SAICAR synthetase antibody
    see all

Images

  • All lanes : Anti-PAICS/PAIS antibody (ab240743) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for PAICS/PAIS using ab240743 at 1 µg/ml in immunohistochemical analysis. DAB staining.

  • PAICS/PAIS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240743 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240743 at 0.4 µg/ml.

    Lane 1: ab240743 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab240743 has not yet been referenced specifically in any publications.

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