• Product name

  • Description

    Rabbit polyclonal to PAICS/PAIS
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human PAICS/PAIS aa 1-50. The exact sequence is proprietary. (NP_001072992.1).
    Database link: P22234

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: PAICS/PAIS in HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
  • General notes

     This product was previously labelled as PAICS




Our Abpromise guarantee covers the use of ab240743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Pathway

    Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
    Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
  • Sequence similarities

    In the N-terminal section; belongs to the SAICAR synthetase family.
    In the C-terminal section; belongs to the AIR carboxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADE2 antibody
    • ADE2H1 antibody
    • AIR carboxylase antibody
    • AIRC antibody
    • DKFZp781N1372 antibody
    • MGC1343 antibody
    • MGC5024 antibody
    • Multifunctional protein ADE2 antibody
    • Multifunctional protein ADE2H1 antibody
    • Paics antibody
    • PAIS antibody
    • Phosphoribosylaminoimidazole carboxylase antibody
    • Phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase antibody
    • PUR6_HUMAN antibody
    • SAICAR synthetase antibody
    see all


  • All lanes : Anti-PAICS/PAIS antibody (ab240743) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Exposure time: 30 seconds

    Lysates prepared using NETN lysis buffer.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for PAICS/PAIS using ab240743 at 1 µg/ml in immunohistochemical analysis. DAB staining.

  • PAICS/PAIS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240743 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240743 at 0.4 µg/ml.

    Lane 1: ab240743 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.


ab240743 has not yet been referenced specifically in any publications.

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