Recombinant
RabMAb

Recombinant Anti-PAIP1 antibody [EPR13258(B)] (ab175211)

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Overview

  • Product name

    Anti-PAIP1 antibody [EPR13258(B)]
    See all PAIP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13258(B)] to PAIP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human PAIP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H074

  • Positive control

    • HeLa, HepG2 and 293T cell lysates.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13258(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.

    • Sequence similarities

      Contains 1 MIF4G domain.

    • Domain

      Only the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • MGC12360 antibody
        • OTTHUMP00000221601 antibody
        • OTTMUSP00000026880 antibody
        • PABC1 interacting protein 1 antibody
        • PABP interacting protein 1 antibody
        • PABP-interacting protein 1 antibody
        • PAIP 1 antibody
        • PAIP-1 antibody
        • Paip1 antibody
        • PAIP1_HUMAN antibody
        • Poly(A) binding protein interacting protein 1 antibody
        • Poly(A)-binding protein-interacting protein 1 antibody
        • Polyadenylate binding protein interacting protein 1 antibody
        • Polyadenylate-binding protein-interacting protein 1 antibody
        see all

      Images

      • Western blot - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
        Western blot - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
        All lanes : Anti-PAIP1 antibody [EPR13258(B)] (ab175211) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 54 kDa

      • Immunoprecipitation - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
        Immunoprecipitation - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)

        Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175211, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      References

      This product has been referenced in:

      • Thakor N  et al. Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation. RNA Biol 14:553-567 (2017). Read more (PubMed: 26828225) »
      See 1 Publication for this product

      Publishing research using ab175211? Please let us know so that we can cite the reference in this datasheet.

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