RecombinantRabMAb

Recombinant Anti-PAIP1 antibody [EPR13258(B)] (ab175211)

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Western blot - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
  • Immunoprecipitation - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
  • Anti-PAIP1 antibody [EPR13258(B)] (ab175211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13258(B)] to PAIP1
  • Suitable for: IP, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PAIP1 antibody [EPR13258(B)]
    See all PAIP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13258(B)] to PAIP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human PAIP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H074

  • Positive control

    • HeLa, HepG2 and 293T cell lysates.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13258(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.

    • Sequence similarities

      Contains 1 MIF4G domain.

    • Domain

      Only the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • MGC12360 antibody
        • OTTHUMP00000221601 antibody
        • OTTMUSP00000026880 antibody
        • PABC1 interacting protein 1 antibody
        • PABP interacting protein 1 antibody
        • PABP-interacting protein 1 antibody
        • PAIP 1 antibody
        • PAIP-1 antibody
        • Paip1 antibody
        • PAIP1_HUMAN antibody
        • Poly(A) binding protein interacting protein 1 antibody
        • Poly(A)-binding protein-interacting protein 1 antibody
        • Polyadenylate binding protein interacting protein 1 antibody
        • Polyadenylate-binding protein-interacting protein 1 antibody
        see all

      Images

      • Western blot - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
        Western blot - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
        All lanes : Anti-PAIP1 antibody [EPR13258(B)] (ab175211) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 54 kDa

      • Immunoprecipitation - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
        Immunoprecipitation - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)

        Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175211, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      • Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
        Anti-PAIP1 antibody [EPR13258(B)] (ab175211)

      References (1)

      Publishing research using ab175211? Please let us know so that we can cite the reference in this datasheet.

      ab175211 has been referenced in 1 publication.

      • Thakor N  et al. Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation. RNA Biol 14:553-567 (2017). PubMed: 26828225

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