Anti-PAIP1 antibody [EPR13258(B)] (ab175211)
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Overview
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Product nameAnti-PAIP1 antibody [EPR13258(B)]
See all PAIP1 primary antibodies -
DescriptionRabbit monoclonal [EPR13258(B)] to PAIP1
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic peptide within Human PAIP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H074 -
Positive control
- HeLa, HepG2 and 293T cell lysates.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR13258(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab175211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/10 - 1/100. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 54 kDa. |
Target
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FunctionActs as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
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Sequence similaritiesContains 1 MIF4G domain.
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DomainOnly the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 10605 Human
- Entrez Gene: 365684 Rat
- Omim: 605184 Human
- SwissProt: Q9H074 Human
- Unigene: 482038 Human
- Unigene: 9450 Rat
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Alternative names
- MGC12360 antibody
- OTTHUMP00000221601 antibody
- OTTMUSP00000026880 antibody
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Images
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![Western blot - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)](https://www.abcam.com/ps/products/175/ab175211/Images/ab175211-201715-ab1752111.JPG)
Western blot - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)All lanes : Anti-PAIP1 antibody [EPR13258(B)] (ab175211) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa -
![Immunoprecipitation - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)](https://www.abcam.com/ps/products/175/ab175211/Images/ab175211-201716-ab1752112.JPG)
Immunoprecipitation - Anti-PAIP1 antibody [EPR13258(B)] (ab175211)Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175211, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
References
ab175211 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"